Sundström C, Radzun H J, Parwaresch M R
J Natl Cancer Inst. 1982 Dec;69(6):1255-60.
Net enzyme activities of normal human blood cells were measured, and isoelectric focusing patterns of acid esterase (AcE) (EC 3.1.1.6) and acid phosphatase (AcP) (EC 3.1.3.2) were compared with corresponding data obtained for two acute T-lymphoblastic leukemia cell lines (JM, Molt-4), one acute B-lymphoblastic leukemia cell line (Ball-1), one acute non-B-non-T-lymphoblastic leukemia cell line (KM 3), and one promyelocytic leukemia cell line (HL-60). The AcE isozymes, found in the individual blood cell types, were regularly expressed by the corresponding cell lines. AcP was regularly expressed by lines HL-60 and KM 3, but lines JM, Molt-4, and Ball-1 showed additional isozymes and/or reduction of the intensity of the typical isozymes found in the presumed normal counterparts. This phenomenon resulted partly in an obscuration of the typical isozyme patterns. Our study documents the applicability of isozyme mapping to the characterization of permanent hematopoietic cell lines. The results suggest that long-term culture conditions can repress phenotypic properties and/or derepress gene activities.
测定了正常人血细胞的净酶活性，并将酸性酯酶（AcE）（EC 3.1.1.6）和酸性磷酸酶（AcP）（EC 3.1.3.2）的等电聚焦图谱与两种急性T淋巴细胞白血病细胞系（JM、Molt-4）、一种急性B淋巴细胞白血病细胞系（Ball-1）、一种急性非B非T淋巴细胞白血病细胞系（KM 3）以及一种早幼粒细胞白血病细胞系（HL-60）所获得的相应数据进行了比较。在各个血细胞类型中发现的AcE同工酶，在相应的细胞系中呈规律性表达。AcP在HL-60和KM 3细胞系中呈规律性表达，但JM、Molt-4和Ball-1细胞系显示出额外的同工酶和/或假定正常对应物中典型同工酶强度的降低。这种现象部分导致了典型同工酶图谱的模糊。我们的研究证明了同工酶图谱在永久性造血细胞系表征中的适用性。结果表明，长期培养条件可抑制表型特性和/或解除基因活性的抑制。
