Wang Qingqing, Li Nan, Wang Xiaojian, Shen Jiangen, Hong Xuejun, Yu Hai, Zhang Yuanyuan, Wan Tao, Zhang Lihuang, Wang Jianli, Cao Xuetao
Institute of Immunology, Zhejiang University, 353 Yan'an Road, Hangzhou 310031, PR China.
Life Sci. 2007 Jan 9;80(5):420-9. doi: 10.1016/j.lfs.2006.09.043. Epub 2006 Oct 14.
We report here the molecular cloning and characterization of a novel human gene (hMYADM) derived from a human bone marrow stromal cell (BMSC) cDNA library, which shares high homology with mouse myeloid-associated differentiation marker (MYADM). hMYADM is also closely related to many other eukaryotic proteins, which together form a novel and highly conserved MYADM-like family. hMYADM with 322-residue protein contains eight putative transmembrane segments and confocal microscopic analysis confirmed its membrane localization by using anti-hMYADM monoclonal antibody. hMYADM mRNA was selectively expressed in human monocytes, dendritic cells, promyeloid or monocytic leukemia cell lines, but not in CD4+, CD8+, CD19+ cells, nor in T cell leukemia or lymphocytic leukemia cell lines. hMYADM expression was also found in normal human bone marrow enriched for CD34+ stem cells, and the expression was up-regulated when these cells were induced to differentiate toward myeloid cells. The mRNA expression level of hMYADM significantly increased in acute promyelocytic leukemia HL-60 and chronic myelogenous leukemia K562 cell line after phorbol myristate acetate (PMA)-induced differentiation. Our study suggests that hMYADM is selectively expressed in myeloid cells, and involved in the myeloid differentiation process, indicating that hMYADM may be one useful membrane marker to monitor stem cell differentiation or myeloid leukemia differentiation.
我们在此报告从人骨髓基质细胞（BMSC）cDNA文库中获得的一个新型人类基因（hMYADM）的分子克隆及特性分析，该基因与小鼠髓系相关分化标志物（MYADM）具有高度同源性。hMYADM还与许多其他真核蛋白密切相关，它们共同构成了一个新颖且高度保守的类MYADM家族。含322个氨基酸残基的hMYADM蛋白含有八个推定的跨膜区段，共聚焦显微镜分析通过使用抗hMYADM单克隆抗体证实了其膜定位。hMYADM mRNA在人单核细胞、树突状细胞、早幼粒细胞或单核细胞白血病细胞系中选择性表达，但在CD4 +、CD8 +、CD19 +细胞中不表达，在T细胞白血病或淋巴细胞白血病细胞系中也不表达。在富含CD34 +干细胞的正常人骨髓中也发现了hMYADM表达，当这些细胞被诱导向髓系细胞分化时，其表达上调。在佛波酯肉豆蔻酸酯乙酸酯（PMA）诱导分化后，急性早幼粒细胞白血病HL - 60和慢性粒细胞白血病K562细胞系中hMYADM的mRNA表达水平显著增加。我们的研究表明，hMYADM在髓系细胞中选择性表达，并参与髓系分化过程，这表明hMYADM可能是监测干细胞分化或髓系白血病分化的一个有用的膜标志物。
