Kawauchi Y, Muto A, Osawa S
Mol Gen Genet. 1982;188(1):7-11. doi: 10.1007/BF00332989.
The whole cell proteins and the ribosomal proteins of Mycoplasma capricolum ATCC 27343 have been analyzed by two-dimensional polyacrylamide gel electrophoresis. The M. capricolum cell is relatively rich in basic proteins. The number of total protein spots detected was approximately 355, which is less than one-third of that of Escherichia coli or Bacillus subtilis. In contrast, the number (30 and 20 protein species have been found to be present in the 50S and 30S ribosomal subunits, respectively) and the size of the ribosomal proteins in the M. capricolum do not seem to be significantly different from those of typical eubacteria.
利用二维聚丙烯酰胺凝胶电泳对山羊支原体ATCC 27343的全细胞蛋白和核糖体蛋白进行了分析。山羊支原体细胞中碱性蛋白相对丰富。检测到的总蛋白斑点数约为355个,不到大肠杆菌或枯草芽孢杆菌的三分之一。相比之下,山羊支原体中核糖体蛋白的数量(分别在50S和30S核糖体亚基中发现有30种和20种蛋白)和大小似乎与典型真细菌的核糖体蛋白没有显著差异。