Nolan J C, Pickett W, English K, Oronsky A L, Kerwar S S
Prostaglandins. 1982 Oct;24(4):443-50. doi: 10.1016/0090-6980(82)90002-8.
The serum-free spent medium of lipopolysaccharide-activated rabbit peritoneal macrophages contains a proteinaceous factor that stimulates the synthesis of PGE2 in rabbit articular chondrocytes. Synthesis of this factor by macrophages is inhibited by cycloheximide. Stimulation of PGE2 in chondrocytes is detected after a four-hour exposure to the macrophage factor and is completely abolished by the addition of either cycloheximide or indomethacin to the chondrocyte cultures. The macrophage derived factor has an apparent molecular weight of 30,000, is heat stable and not inactivated upon reductive alkylation or on treatment with phenylglyoxal. Activity is partially destroyed upon treatment with acid (pH 2.0) and upon trypsin treatment.
脂多糖激活的兔腹膜巨噬细胞的无血清用过的培养基含有一种蛋白质因子，该因子可刺激兔关节软骨细胞中前列腺素E2（PGE2）的合成。巨噬细胞对该因子的合成受环己酰亚胺抑制。软骨细胞在暴露于巨噬细胞因子4小时后可检测到PGE2的刺激，并且通过向软骨细胞培养物中添加环己酰亚胺或吲哚美辛可完全消除这种刺激。巨噬细胞衍生因子的表观分子量为30,000，热稳定，在还原烷基化或用苯乙二醛处理后不会失活。用酸（pH 2.0）处理和用胰蛋白酶处理后，活性会部分被破坏。
