Nolan J C, Pickett W C
Agents Actions. 1985 Oct;17(1):73-6. doi: 10.1007/BF01966685.
Recent work from this laboratory has shown that macrophages in culture synthesize and secrete a soluble factor(s) that induces the synthesis of collagenase in primary cultures of rabbit chondrocytes (Arth. Rheum. 23, 448, 1980). The current studies were undertaken to determine the role of arachidonate metabolism in this process. Incubation of chondrocytes with MCM (Macrophage Conditioned Medium) and low doses of indomethacin (1-10 microM) had no effect on collagenase synthesis. The lipoxygenase inhibitor NDGA, indomethacin at high doses (50 microM), diethylcarbamazine and the phospholipase inhibitor dibromoacetophenone, inhibited the MCM dependent synthesis of collagenase in chondrocytes. These inhibitors did not affect collagenase activity nor did they interfere with the activation of latent collagenase. Our data indicate that although cyclooxygenase plays no role in the MCM dependent induction of collagenase in chondrocytes, lipoxygenase activity may be essential.
该实验室最近的研究表明，培养中的巨噬细胞合成并分泌一种可溶性因子，可诱导兔软骨细胞原代培养物中胶原酶的合成（《关节炎与风湿病》23卷，448页，1980年）。目前的研究旨在确定花生四烯酸代谢在此过程中的作用。用巨噬细胞条件培养基（MCM）和低剂量消炎痛（1 - 10微摩尔）孵育软骨细胞对胶原酶合成没有影响。脂氧合酶抑制剂NDGA、高剂量消炎痛（50微摩尔）、二乙氨基甲嗪和磷脂酶抑制剂二溴苯乙酮，均可抑制软骨细胞中依赖MCM的胶原酶合成。这些抑制剂不影响胶原酶活性，也不干扰潜在胶原酶的激活。我们的数据表明，虽然环氧化酶在软骨细胞中依赖MCM诱导胶原酶的过程中不起作用，但脂氧合酶活性可能至关重要。
