Evêquoz V, Schnyder J, Trechsel U, Baggiolini M, Fleisch H
Biochem J. 1984 Apr 15;219(2):667-77. doi: 10.1042/bj2190667.
We describe the effects of products of mononuclear phagocytes on the secretory activity of chondrocytes. The primary confluent cultures of rabbit articular chondrocytes were exposed to standard medium alone or enriched with conditioned medium obtained from cultures of rabbit peritoneal macrophages, the mouse macrophage cell line P388D1 or human blood mononuclear cells. Four markers of release were assessed, the neutral proteinases plasminogen activator and collagenase, the acid hydrolase beta-glucuronidase and prostaglandin E2, and the kinetics of their changes were monitored. Chondrocytes that were cultured in standard medium secreted large amounts of plasminogen activator, some beta-glucuronidase, but no collagenase, and released only minor amounts of prostaglandin E2. The addition of conditioned medium from rabbit macrophages induced a rapid release of large quantities of prostaglandin E2 and an abundant secretion of collagenase, while abolishing or strongly decreasing plasminogen activator secretion. In addition, beta-glucuronidase secretion was markedly enhanced. The decrease in secretion of plasminogen activator appeared to reflect a diminished production, since no evidence was found for the generation of inhibitors or for an accelerated extracellular breakdown of the enzyme. Conditioned media of the mouse and human mononuclear cells influenced the secretory activities of rabbit articular chondrocytes in a similar way, suggesting that the factor (or factors) acting on chondrocytes is produced by a variety of macrophages, and that its action is not species-restricted. The time course and concentration-dependence of the effects observed indicate that the secretion of plasminogen activator and collagenase are influenced in a strictly reciprocal fashion by the macrophage products. The release of prostaglandin E2 paralleled that of collagenase.
我们描述了单核吞噬细胞产物对软骨细胞分泌活性的影响。将兔关节软骨细胞的原代汇合培养物分别置于单独的标准培养基中，或添加从兔腹膜巨噬细胞、小鼠巨噬细胞系P388D1或人血单核细胞培养物中获得的条件培养基。评估了四种释放标志物，即中性蛋白酶纤溶酶原激活剂和胶原酶、酸性水解酶β-葡萄糖醛酸酶和前列腺素E2，并监测了它们变化的动力学。在标准培养基中培养的软骨细胞分泌大量纤溶酶原激活剂、少量β-葡萄糖醛酸酶，但不分泌胶原酶，仅释放少量前列腺素E2。添加兔巨噬细胞的条件培养基可诱导大量前列腺素E2的快速释放和胶原酶的大量分泌，同时消除或强烈减少纤溶酶原激活剂的分泌。此外，β-葡萄糖醛酸酶的分泌明显增强。纤溶酶原激活剂分泌的减少似乎反映了其产生的减少，因为未发现有抑制剂产生或该酶细胞外加速降解的证据。小鼠和人单核细胞的条件培养基以类似方式影响兔关节软骨细胞的分泌活性，这表明作用于软骨细胞的因子是由多种巨噬细胞产生的，且其作用不受物种限制。观察到的效应的时间进程和浓度依赖性表明，纤溶酶原激活剂和胶原酶的分泌受到巨噬细胞产物的严格相互影响。前列腺素E2的释放与胶原酶的释放平行。
