Corvol M T, Dumontier M F, Rappaport R, Guyda H, Posner B I
Acta Endocrinol (Copenh). 1978 Oct;89(2):263-75. doi: 10.1530/acta.0.0890263.
Chondrocyte cultures were prepared from rabbit growth plate (GPC) and articular (ARC) chondrocytes. These two cell types have distinct morphological characteristics. The cells reached maximum numbers by days 10 and 21 for ARC and GPC, respectively. The proteoglycans (PG) contained in the cellular pool were extracted and purified by DEAE cellulose chromatography. The effect of a partially purified somatomedin peptide with insulin-like activity on [35S]sulphate incorporation into PG was evaluated. In both ARC and GPC a significant stimulation of [35S]sulphate uptake into PG subunits was obtained with 1 ng Eq./ml of somatomedin peptide. In order to obtain the same stimulatory effect with porcine insulin, a 1000-fold greater concentration was required. The electrophoretic patterns of the PG subunits on acrylamide-agarose electrophoresis were identical on control incubations and after stimulation with the somatomedin peptide. These data demonstrate in vitro biological activity of this peptide on well differentiated articular and epiphyseal growth plate chondrocytes in culture. These cultures appear to provide a sensitive biological assay for somatomedin peptides.
软骨细胞培养物取自兔生长板（GPC）软骨细胞和关节（ARC）软骨细胞。这两种细胞类型具有明显不同的形态特征。ARC和GPC细胞分别在第10天和第21天达到最大数量。通过DEAE纤维素色谱法提取并纯化细胞池中所含的蛋白聚糖（PG）。评估了具有胰岛素样活性的部分纯化的生长调节素肽对[35S]硫酸盐掺入PG的影响。在ARC和GPC中，1 ng Eq./ml的生长调节素肽均能显著刺激[35S]硫酸盐摄取到PG亚基中。为了用猪胰岛素获得相同的刺激效果，则需要高1000倍的浓度。在对照孵育以及用生长调节素肽刺激后，PG亚基在丙烯酰胺-琼脂糖电泳上的电泳图谱相同。这些数据证明了该肽在体外对培养的分化良好的关节和骨骺生长板软骨细胞具有生物学活性。这些培养物似乎为生长调节素肽提供了一种灵敏的生物学检测方法。
