Ashton I K, Spencer E M
Early Hum Dev. 1983 Jul;8(2):135-40. doi: 10.1016/0378-3782(83)90069-5.
Partly purified somatomedin prepared from adult human plasma stimulated [3H]thymidine and [35S]sulphate uptake into human cartilage. At a final concentration of 0.1 U/ml somatomedin the uptake of [3H]thymidine into fetal cartilage at 18 weeks of gestation was 162 +/- 23% compared with controls. Somatomedin also increased the incorporation of [35S]sulphate into fetal cartilage to 137 +/- 22% at 18 weeks compared to controls. The same concentration of somatomedin (0.1 U/ml) also increased the incorporation of [3H]thymidine into postnatal articular cartilage to 239 +/- 69% and [35S]sulphate to 133 +/- 28% compared to controls. Human somatomedin prepared from adult plasma thus has a mitogenic effect on both fetal and postnatal cartilage. The smaller effect on fetal cartilage may reflect different forms of somatomedin and/or differences in receptors for somatomedins on adult and fetal chondrocytes.
从成人血浆中制备的部分纯化的生长调节素刺激了[3H]胸腺嘧啶核苷和[35S]硫酸盐进入人软骨。在生长调节素终浓度为0.1 U/ml时，与对照组相比，妊娠18周时胎儿软骨对[3H]胸腺嘧啶核苷的摄取为162±23%。生长调节素还使妊娠18周时胎儿软骨中[35S]硫酸盐的掺入量与对照组相比增加到137±22%。相同浓度的生长调节素（0.1 U/ml）与对照组相比，也使出生后关节软骨中[3H]胸腺嘧啶核苷的掺入量增加到239±69%，[35S]硫酸盐增加到133±28%。因此，从成人血浆中制备的人生长调节素对胎儿和出生后的软骨都有促有丝分裂作用。对胎儿软骨作用较小可能反映了生长调节素的不同形式和/或成人及胎儿软骨细胞上生长调节素受体的差异。
