Corvol M T, Dumontier M F, Maroteaux P, Rappaport R, Guyda M, Posner B
Arch Fr Pediatr. 1978 Dec;35(10 Suppl):57-64.
Chondrocytes from normal cartilage of a 2 month old child have been cultured. In vitro the cells maintained their morphological identity and synthesised proteoglycans with electrophoretic properties similar to those synthesised by cartilage in vivo. Sulphation was stimulated by vitamin D metabolites and by somatomedin. This technique has been used to study biopsies of abnormal cartilage.
已对一名2个月大儿童正常软骨中的软骨细胞进行了培养。在体外,这些细胞保持了它们的形态特征,并合成了具有与体内软骨合成的蛋白聚糖相似电泳特性的蛋白聚糖。维生素D代谢产物和生长调节素刺激了硫酸化作用。该技术已被用于研究异常软骨的活检样本。