人牙菌斑细菌突变体粘性放线菌T14Av细胞生长和细胞外粘液多糖产生的最低营养需求

Minimum nutritional requirements for cellular growth and extracellular slime polysaccharide production by the human dental plaque bacterial mutant Actinomyces viscosus T14Av.

作者信息

Ooshima T, Kuramitsu H K

出版信息

Arch Oral Biol. 1982;27(10):847-52. doi: 10.1016/0003-9969(82)90040-1.

Abstract

The requirements were examined in chemically-defined media. Magnesium sulphate, potassium phosphate and adenine were essential for cellular growth and sodium bicarbonate and inositol further stimulated growth. The addition of sodium phosphate to this medium stimulated both cellular growth and slime production. In contrast, ammonium sulphate inhibited slime production. Biotin and thiamine stimulated cellular growth and nicotinic acid was essential for slime production. Glutamine, alanine and cystine were essential for cellular growth. A minimal medium which supported both good cellular growth and slime production for strain T14Av contained adenine, sodium bicarbonate, MgSO4, inositol, glucose, KH2PO4, Na2HPO4, biotin, thiamine, nicotinic acid, arginine, glutamine, alanine and cystine.

摘要

在化学成分明确的培养基中对各项需求进行了研究。硫酸镁、磷酸钾和腺嘌呤对细胞生长至关重要，碳酸氢钠和肌醇能进一步促进生长。向该培养基中添加磷酸钠可同时促进细胞生长和黏液产生。相比之下，硫酸铵会抑制黏液产生。生物素和硫胺素促进细胞生长，烟酸对黏液产生至关重要。谷氨酰胺、丙氨酸和胱氨酸对细胞生长至关重要。一种能支持T14Av菌株良好细胞生长和黏液产生的基本培养基包含腺嘌呤、碳酸氢钠、硫酸镁、肌醇、葡萄糖、磷酸二氢钾、磷酸氢二钠、生物素、硫胺素、烟酸、精氨酸、谷氨酰胺、丙氨酸和胱氨酸。