Imai S, Kuramitsu H
Infect Immun. 1983 Mar;39(3):1059-66. doi: 10.1128/iai.39.3.1059-1066.1983.
The human isolates Actinomyces viscosus T14V and T14Av produced extracellular polysaccharides in the absence of sucrose. In contrast to strain T14V, strain T14Av produced abundant viscous slime polysaccharide in the culture supernatant fluids when grown in a chemically defined medium containing glucose. After resolution of the T14Av polysaccharides into seven fractions, it was demonstrated that two of these exhibited viscous properties and lacked methylpentose and muramic acid. The major slime polysaccharide purified by gel filtration and ion-exchange column chromatography contained 39% (moles percent carbohydrate) galactose, 37% N-acetylglucosamine, 19% glucose, and 5% mannose. Only trace amounts of protein and phosphorus were detected in this preparation. On the other hand, strain T14V produced negligible slime polysaccharide under the same culture conditions. The major extracellular polysaccharide fraction from this strain contained methylpentoses, hexoses, hexosamines, muramic acid, protein, and phosphorus, suggesting that this fraction might be derived from the cell wall.
人源分离株黏性放线菌T14V和T14Av在没有蔗糖的情况下产生细胞外多糖。与T14V菌株不同，T14Av菌株在含有葡萄糖的化学限定培养基中生长时，在培养上清液中产生大量黏性黏液多糖。将T14Av多糖分离为七个组分后，证明其中两个组分具有黏性，且缺乏甲基戊糖和胞壁酸。通过凝胶过滤和离子交换柱色谱法纯化的主要黏液多糖含有39%（碳水化合物摩尔百分比）半乳糖、37% N-乙酰葡糖胺、19%葡萄糖和5%甘露糖。在该制剂中仅检测到痕量的蛋白质和磷。另一方面，T14V菌株在相同培养条件下产生的黏液多糖可忽略不计。该菌株的主要细胞外多糖组分含有甲基戊糖、己糖、己糖胺、胞壁酸、蛋白质和磷，表明该组分可能源自细胞壁。
