Litwin J, Enzell C, Pilotti A
Acta Pathol Microbiol Scand A. 1978 Mar;86(2):135-41. doi: 10.1111/j.1699-0463.1978.tb02025.x.
Human embryonic diploid lung fibroblasts were exposed to various fractions of cigarette smoke condensate over their in vitro life-time. Most fractions were toxic at a concentration of 50 microgram/ml, with the exception of the strong acid and one water soluble basic fraction, which stimulated growth and increased longevity significantly at this concentration. Most fractions produced no effect on cell growth at 10 microgram/ml, with the exception of another basic fraction which inhibited growth at 1 microgram/ml. Nicotine had no apparent effect on growth and longevity at 50 microgram/ml. The neutral fraction containing the polynuclear hydrocarbon carcinogens produced normal growth and longevity at 10 microgram/ml. No cell transformations were observed.
人胚二倍体肺成纤维细胞在其体外存活期内暴露于香烟烟雾浓缩物的不同组分中。大多数组分在浓度为50微克/毫升时具有毒性，但强酸组分和一种水溶性碱性组分除外，这两种组分在此浓度下能显著刺激生长并延长细胞寿命。大多数组分在10微克/毫升时对细胞生长无影响，但另一种碱性组分除外，它在1微克/毫升时会抑制生长。尼古丁在50微克/毫升时对生长和寿命无明显影响。含有多核烃致癌物的中性组分在10微克/毫升时能使细胞正常生长并具有正常寿命。未观察到细胞转化现象。
