Bronckers A L, Zorge J A, Wöltgens J H
J Biol Buccale. 1982 Dec;10(4):263-9.
The dose response relationship between medium concentrations of vitamin C (0-350 micrograms/ml) and the hydroxylation of in vitro incorporated 3H-proline showed that in (small) 2nd molars 50 micrograms/ml vit C at level of a maximum hydroxylation was attained at whereas in (larger) 1st molars higher medium concentrations seemed to be required (150 micrograms/ml or more). No cytotoxic effects were observed in media containing concentrations of vit C up to 350 micrograms/ml. In the absence of vit C odontoblasts in young 2nd molars failed to differentiate normally and an abnormal jelly-like substance was produced. Dentinogenesis in the 2nd molars was normal in the presence of at least 50 micrograms/ml vit C. In the 1st molars, in which production of collagenous dentine matrix had already started at the time of explantation, significantly less 3H-hydroxyproline was measured during vit C deficiency as early as the first day of culture, suggesting that there is a high need for vit C during dentinogenesis in vitro.
中等浓度维生素C（0 - 350微克/毫升）与体外掺入的3H - 脯氨酸羟基化之间的剂量反应关系表明，在（较小的）第二磨牙中，达到最大羟基化水平时维生素C的浓度为50微克/毫升，而在（较大的）第一磨牙中，似乎需要更高的中等浓度（150微克/毫升或更高）。在维生素C浓度高达350微克/毫升的培养基中未观察到细胞毒性作用。在缺乏维生素C的情况下，年轻第二磨牙中的成牙本质细胞无法正常分化，并产生异常的果冻状物质。在至少含有50微克/毫升维生素C的情况下，第二磨牙的牙本质形成正常。在第一磨牙中，在植入时胶原牙本质基质的产生就已经开始，早在培养的第一天，在维生素C缺乏期间测得的3H - 羟脯氨酸就明显减少，这表明在体外牙本质形成过程中对维生素C的需求量很大。
