Fc受体在人和大鼠恶性组织中的定位

Localization of Fc receptors in human and rat malignant tissues.

作者信息

Tönder O, Krishnan E C, Morse P A, Jewell W R, Humphrey L J

出版信息

Acta Pathol Microbiol Scand C. 1978 Aug;86C(4):173-8. doi: 10.1111/j.1699-0463.1978.tb02576.x.

DOI:10.1111/j.1699-0463.1978.tb02576.x

Abstract

Three human malignant tumors and a rat carcinoma (Walker 256), which all showed strong Fc receptor activity in tests with sheep erythrocytes (E) sensitized with rabbit IgG antibodies (A), were used to study the distribution of the receptor in subcellular fractions isolated by differential centrifugation. Smears of the three crude sediments obtained (nuclei, mitochondria rich and membrane rich) all possessed receptor activity. EA incubated in the suspension of cell fractions did not attach to Fc receptor positive tissue sections or to cells in suspension. Cells from which the surface receptors had been eluted still showed activity in sections of cell pellets. Apparently the Fc activity is connected with both external and internal membranes.

摘要

三种人类恶性肿瘤和一种大鼠癌（Walker 256），在用兔IgG抗体（A）致敏的绵羊红细胞（E）进行的试验中均显示出强烈的Fc受体活性，被用于研究通过差速离心分离的亚细胞组分中受体的分布。所获得的三种粗沉淀物（细胞核、富含线粒体和富含膜）的涂片均具有受体活性。在细胞组分悬浮液中孵育的EA不附着于Fc受体阳性组织切片或悬浮细胞。表面受体已被洗脱的细胞在细胞沉淀切片中仍显示活性。显然，Fc活性与外部和内部膜均有关联。