Lilius E M, Marnila P
Department of Biochemistry, University of Turku, Finland.
Experientia. 1992 Dec 1;48(11-12):1082-91. doi: 10.1007/BF01947995.
Phagocytes, the first-line cells of the body's defence mechanisms against invading pathogens, kill microorganisms by means of lysosomal degradative enzymes and highly toxic reactive oxygen intermediates. The reactive oxygen compounds are produced, in a process called the 'respiratory burst', by the NADPH oxidase complex in plasma membranes, and by myeloperoxidase in phagolysosomes after degranulation. These processes generate electronically excited states which, on relaxation, emit photons, giving rise to phagocyte chemiluminescence (CL). This paper describes the conditions for the measurement of CL, and reviews the activity of phagocytes from individuals undergoing stress or disease. The capability of phagocytes to emit photons reflects remarkably well the pathophysiological state of the host. In many cases even the magnitude of the stress, the presence of a pathogen in the body, or the activity of the disease can be estimated. Physiological changes, e.g. in the reproductive cycle, can also be predicted.
吞噬细胞是机体抵御入侵病原体防御机制的一线细胞，它们通过溶酶体降解酶和高毒性活性氧中间体来杀死微生物。活性氧化合物是在一个称为“呼吸爆发”的过程中产生的，由质膜中的NADPH氧化酶复合物以及脱颗粒后吞噬溶酶体中的髓过氧化物酶产生。这些过程产生电子激发态，在弛豫时会发射光子，从而产生吞噬细胞化学发光（CL）。本文描述了测量CL的条件，并综述了处于应激或疾病状态个体的吞噬细胞活性。吞噬细胞发射光子的能力很好地反映了宿主的病理生理状态。在许多情况下，甚至可以估计应激的程度、体内病原体的存在或疾病的活动情况。生理变化，例如生殖周期中的变化，也可以被预测。
