Banderet E, Despont J P
Int Arch Allergy Appl Immunol. 1980;61(1):1-8. doi: 10.1159/000232408.
Peripheral blood lymphocytes from patients with chronic lymphatic leukaemia (CLL) were compared to normal purified B lymphocytes. Lymphocytes were cultured in the presence of 3H-fucose, and glycoproteins isolated by lentil lectin, were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Most of the major components detected in normal B lymphocytes were also present in CLL lymphocytes. Although B and CLL lymphocytes shared several components of similar molecular size, the latter appeared to be a heterogeneous cell population. The most important finding was the presence of a prominent component of approximately component of approximately 35,000 daltons in normal B and in 7 out of 8 cases of CLL. This component was not related to surface immunoglobulins but appeared to have a molecular weight very similar to the human Ia-like antigen. A labelled component of approximately 140,000 daltons was also present in large amounts in B lymphocytes and in 5 cases of CLL. Receptors specific for CLL lymphocytes were not found.
将慢性淋巴细胞白血病（CLL）患者的外周血淋巴细胞与正常纯化的B淋巴细胞进行比较。淋巴细胞在3H-岩藻糖存在的情况下进行培养，通过扁豆凝集素分离的糖蛋白，采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳进行分析。在正常B淋巴细胞中检测到的大多数主要成分在CLL淋巴细胞中也存在。虽然B淋巴细胞和CLL淋巴细胞共有几种分子大小相似的成分，但后者似乎是一个异质性细胞群体。最重要的发现是，在正常B淋巴细胞以及8例CLL患者中的7例中，存在一种约35,000道尔顿的突出成分。该成分与表面免疫球蛋白无关，但分子量似乎与人Ia样抗原非常相似。一种约140,000道尔顿的标记成分在B淋巴细胞和5例CLL患者中也大量存在。未发现CLL淋巴细胞特有的受体。
