Curtis J L, Nordin A A
Immunology. 1984 Aug;52(4):711-9.
The specific primary in vitro plaque-forming cell (PFC) response of C57B1/6 nu/nu spleen cells to the Type 2 T-independent (TI-2) antigen DAGG-Ficoll was analysed in the absence of T cell help in a serum-free medium. Lipopolysaccharide (LPS) enhancement of the antigen-specific response was shown to be mediated by soluble factors contained in the supernatants of LPS-induced bone marrow-derived macrophages. The activity of these supernatants was not associated with residual LPS, since the antigen-specific response of B cells from mice genetically deficient in LPS receptors was equally well enhanced. The activity of these supernatants was associated with interleukin-1 (IL-1) and partially purified IL-1 prepared from P388D1 cells also enhanced the primary in vitro response to DAGG-Ficoll. Limiting dilution analysis experiments showed that only in the presence of exogenously added IL-1 could a single cell type, presumably the B cells, be shown to be limiting.
在无血清培养基中，在无T细胞辅助的情况下，分析了C57B1/6裸鼠脾细胞对2型非T细胞依赖性（TI-2）抗原DAGG-菲可的特异性体外空斑形成细胞（PFC）反应。脂多糖（LPS）对抗原特异性反应的增强作用被证明是由LPS诱导的骨髓来源巨噬细胞上清液中所含的可溶性因子介导的。这些上清液的活性与残留的LPS无关，因为来自LPS受体基因缺陷小鼠的B细胞的抗原特异性反应同样得到了很好的增强。这些上清液的活性与白细胞介素-1（IL-1）有关，从P388D1细胞制备的部分纯化的IL-1也增强了对DAGG-菲可的体外初次反应。有限稀释分析实验表明，只有在外源添加IL-1的情况下，一种单一的细胞类型，可能是B细胞，才被证明是限制性的。
