Niskanen E, Ashman R, Cline M J
J Lab Clin Med. 1980 Jun;95(6):934-42.
Erythroid colony formation was enhanced by treatment of mouse bone marrow cells with PHA prior to culture in diffusion chambers implanted into intact mice. Addition of PHA-pretreated thymocytes, lymph node lymphocytes, and spleen cells to untreated bone marrow cells also resulted in increased numbers of erythroid colonies in diffusion chambers but had no effect on myeloid colonies. PHA did not augment erythroid colony formation when bone marrow was depleted of Thy 1-bearing cells, although cells capable of erythroid differentiation were still present as judged by the response to erythropoietin. Furthermore, mixing of bone marrow depleted of Thy 1 + cells with PHA-treated thymocytes restored augmented erythroid colony formation. Lack of responsiveness of bone marrow cells to concanavalin A and pokeweed mitogen may indicate that a specific T cell subpopulation reacting with PHA is able to stimulate erythropoiesis. Note that addition of untreated thymocytes, lymph node lymphocytes, and spleen cells into cultures had no effect.
在用植入完整小鼠体内的扩散小室培养之前，用PHA处理小鼠骨髓细胞可增强红系集落形成。将经PHA预处理的胸腺细胞、淋巴结淋巴细胞和脾细胞添加到未处理的骨髓细胞中，也会导致扩散小室中红系集落数量增加，但对髓系集落没有影响。当骨髓中Thy 1阳性细胞耗尽时，PHA不会增强红系集落形成，尽管根据对促红细胞生成素的反应判断，仍存在能够进行红系分化的细胞。此外，将耗尽Thy 1 +细胞的骨髓与经PHA处理的胸腺细胞混合，可恢复增强的红系集落形成。骨髓细胞对刀豆球蛋白A和商陆有丝分裂原无反应，这可能表明与PHA反应的特定T细胞亚群能够刺激红细胞生成。注意，将未处理的胸腺细胞、淋巴结淋巴细胞和脾细胞添加到培养物中没有效果。
