Carnaud C, Altman J, Errasti P, Van der Gaag R
Scand J Immunol. 1980;11(5):503-10. doi: 10.1111/j.1365-3083.1980.tb00018.x.
The in vivo immunization of mice with human lymphoblastoid cell line LNH13 generates direct cell-mediated cytotoxicity by spleen cells. The lytic activity appears as early as day 3 after the intraperitoneal inoculation of 7.5 x 10(6) cells and persists at least until day 11. The killer cells do not adhere to plastic and are not retained on nylon wool columns or on Degalan beads coated with mouse Ig plus rabbit-anti-mouse Ig. The effector cells are partly inhibited by treatment with anti-Thy-1.2 serum plus complement, but this inhibition appears to be non-specific since anti-serum alone or normal serum plus complement have the same effects. Heat-aggregated IgG strongly inhibits cytotoxicity, indicating that the effector cells are Fc-positive and that such receptors are implicated in lysis. Altogether, these features strongly argue for an ADCC phenomenon. The involvement of antibodies is demonstrated by the fact that eluates (56 degrees C, 30 min) from immune cells alone induce lysis in the presence of normal spleen cells as effectors. The lytic activity of these eluates can be removed by specific adsorption on protein A coupled to Sepharose beads and on the human lymphoid target cells. Positive complementation between immune and non-immune spleen cells suggest that the arming process may occur in vitro during the assay, when antibodies are released by plasmacytes.
用人淋巴母细胞系LNH13对小鼠进行体内免疫，可使脾细胞产生直接的细胞介导细胞毒性。在腹腔接种7.5×10⁶个细胞后，溶细胞活性最早在第3天出现，并至少持续到第11天。杀伤细胞不粘附于塑料，也不能保留在尼龙毛柱上或包被有小鼠Ig加兔抗小鼠Ig的德加兰珠上。效应细胞经抗Thy-1.2血清加补体处理后部分受到抑制，但这种抑制似乎是非特异性的，因为单独的抗血清或正常血清加补体也有相同的作用。热聚集的IgG强烈抑制细胞毒性，表明效应细胞是Fc阳性的，且此类受体参与细胞溶解。总之，这些特征有力地支持了一种ADCC现象。抗体的参与可通过以下事实得到证明：仅来自免疫细胞的洗脱液（56℃，30分钟）在存在正常脾细胞作为效应细胞时可诱导细胞溶解。这些洗脱液的溶细胞活性可通过特异性吸附在偶联于琼脂糖珠的蛋白A和人淋巴样靶细胞上而去除。免疫脾细胞和非免疫脾细胞之间的阳性互补表明，当浆细胞释放抗体时，武装过程可能在检测过程中于体外发生。
