Bardos P, Altman J, Guillou P J, Carnaud C
Clin Exp Immunol. 1981 Mar;43(3):534-9.
A cold target inhibition assay has been used to investigate the relationship between the effector cells responsible for NK and ADCC activity. Two models of ADCC have been studied in parallel with NK, one involving in vivo armed effector cells and the other involving normal effector spleen cells tested on antibody-precoated target cells. Bivalent effector cells active in ADCC and NK as well as monovalent NK cells and monovalent FcR-positive ADCC killer cells appear to exist concomitantly. However, the variability from experiment to experiment of the blocking efficiency displayed by the heterologous cold targets, suggests that the proportions of monovalent to bivalent cells may vary between batches of mice depending on their immunological status at the time of the test. This should be taken into careful consideration when interpreting the results of this type of investigation.
已采用冷靶抑制试验来研究负责自然杀伤（NK）和抗体依赖的细胞介导的细胞毒性（ADCC）活性的效应细胞之间的关系。已与NK平行研究了两种ADCC模型，一种涉及体内武装效应细胞，另一种涉及在抗体预包被的靶细胞上测试的正常效应脾细胞。在ADCC和NK中具有活性的二价效应细胞以及单价NK细胞和单价FcR阳性ADCC杀伤细胞似乎同时存在。然而，异源冷靶所显示的阻断效率在不同实验之间存在变异性，这表明单价细胞与二价细胞的比例可能因小鼠批次而异，具体取决于测试时它们的免疫状态。在解释这类研究结果时应仔细考虑这一点。
