Ryĭgas E M, Sardis Kh Ia, Kaal' V A
Parazitologiia. 1980 Aug;14(4):354-7.
For purifying the cultures of Trichomonas vaginalis and T. hominis from associating yeasts we inoculated them into the upper layer of a viscous medium containing 0.1% agar (Teras, 1955; Tompel, Teras, 1976) poured into a burette. On the 2nd or 3rd day after inoculating the first drops obtained from the lower end of the burette contained as a rule only trichomonads. For purifying the cultures of T. tenax from associating yeasts we prepared a special semisolid medium from the liquid egg medium (Wantland e. a., 1963) by adding agar (0.5%), levorine (50--600 units/1 ccm) and some pieces of solid egg medium (Hallmann, 1953). The curve of an U-tube was filled with this medium and into both branches pure liquid egg medium was poured. In a few days after inoculating the culture of T. tenax with yeasts into one branch of the tube we obtained from the other branch of the tube trichomonads without yeasts.
为了从混合的酵母菌中纯化阴道毛滴虫和人毛滴虫的培养物，我们将它们接种到倒入滴定管中的含有0.1%琼脂的粘性培养基上层（Teras，1955年；Tompel，Teras，1976年）。接种后第2天或第3天，从滴定管下端获得的最初几滴通常只含有滴虫。为了从混合的酵母菌中纯化口腔毛滴虫的培养物，我们在液体鸡蛋培养基（Wantland等人，1963年）中加入琼脂（0.5%）、左旋霉素（50 - 600单位/1立方厘米）和一些固体鸡蛋培养基块（Hallmann，1953年），制备了一种特殊的半固体培养基。用这种培养基填充U形管的曲线部分，并在两个分支中倒入纯液体鸡蛋培养基。将口腔毛滴虫与酵母菌的混合培养物接种到管的一个分支几天后，我们从管的另一个分支中获得了不含酵母菌的滴虫。
