[Kinetic study of neutrophil and macrophage cell reproduction and differentiation in the common frog at different seasons of the year].

A supposed life-span of hemosiderin-containing macrophages in the frog spleen has been described on the basis of their morphology, changes in the number of nuclei, and results of autoradiographic studies of DNA synthesis in various seasons. The hibernating stages of the lines are hemocytoblasts, mononuclear and moderately polynuclear macrophages, which renew the phagocytosis and nuclear division at the beginning of spring. A new population of monomuclear macrophages develops from hemocytoblasts during spring. Large polynuclear forms appear during spring and summer, reach their maximal size and erythrophagocytotic activity towards the end of summer, and die in winter. The most part of the stored pigments is removed from the spleen. DNA synthesis and division occur asynchronously in the nuclei of one cell. Some pathologic forms of macrophages are described. The similarity in the proliferation cell kinetics of neutrophilic and macrophagal lines confirms a close relation between the two. The role of temperature and photoperiod in the regulations of proliferative activity of these cells during spring is discussed.