Cheredeev A N, Grunov R, Koval'chuk L V
Biull Eksp Biol Med. 1980 Jul;89(7):70-3.
Some mechanisms were studied that are concerned with mouse red blood cell rosette formation by human peripheral blood lymphocytes. Lymphocytes rosetting with mouse red blood cells (EM--RFC) were identified by the test similar to the E-rosette formation technique. EM--RFC accounted for 7.7+/-0.48% in the peripheral blood of healthy donors. It has been established by stepwise selective inhibition of Fc- and C3-receptors and surface immunoglobulins that human Fc- and C3-receptors do not take part in rosetting with mouse red blood cells, whilst surface immunoglobulins, particularly IgM or near-located structures, are implicated in the reaction of lymphocytes with mouse red blood cells. Multiple analysis of lymphocyte receptors has been performed in patients with different immunologic disorders. A significant decrease in the EM--RFC level was found in patients with rheumatoid arthritis. In patients with dys-and hypoimmunoglobulinemia, the content of EM--RFC varied depending on the severity of immunity B-system injury and correlated well with the cells bearing surface immunoglobulins. The drastically increased number EM--RFC (up to 34.6--78%) was recorded in the blood of patients with chronic lympholeukemia. These findings served as basis for applying EM--RFC detection for diagnosing various forms of lympholeukemia.
对一些与人类外周血淋巴细胞形成小鼠红细胞花环有关的机制进行了研究。通过类似于E花环形成技术的试验鉴定了与小鼠红细胞形成花环的淋巴细胞（EM-RFC）。健康供体外周血中EM-RFC占7.7±0.48%。通过对Fc和C3受体以及表面免疫球蛋白的逐步选择性抑制已证实，人类Fc和C3受体不参与与小鼠红细胞的花环形成，而表面免疫球蛋白，特别是IgM或其附近结构，与淋巴细胞和小鼠红细胞的反应有关。对患有不同免疫紊乱的患者进行了淋巴细胞受体的多项分析。在类风湿性关节炎患者中发现EM-RFC水平显著降低。在免疫球蛋白异常和免疫球蛋白低下的患者中，EM-RFC的含量根据免疫B系统损伤的严重程度而变化，并且与带有表面免疫球蛋白的细胞密切相关。在慢性淋巴细胞白血病患者的血液中记录到EM-RFC数量急剧增加（高达34.6%-78%）。这些发现为应用EM-RFC检测诊断各种形式的淋巴细胞白血病奠定了基础。
