Kano K, Yoshida H, Yata J, Suda T
Endocrinol Jpn. 1980 Apr;27(2):215-21. doi: 10.1507/endocrj1954.27.215.
Serum concentrations of 25-hydroxyvitamin D (25-OH-D) and 24,25-dihydroxy-vitamin D [24,25-(OH)2-D] were measured in lipid extracts of 1 ml of human sera by a competitive protein binding assay. 25-OH-D and 24,25-(OH)2-D were isolated from th lipid extracts by Sephadex LH-20 column chromatography. The 24,25-(OH)2-D fraction on the Sephadex LH-20 columns were further purified by high pressure liquid chromatography prior to assay. Serum levels of 25-OH-D and 24,25-(OH)2-D in normal males were similar to those in normal females throughout each age group. Serum levels of 25-OH-D and 24,25 (OH)2-D in newborns were 8.28 +/- 1.68 ng/ml (mean +/- SD) and 0.55 +/- 0.16 ng/ml, respectively, which were significantly (p<0.001) lower than those in adults (21.3 +/- 4.8 ng/ml of 25-OH-D and 1.55 +/- 0.31 ng/ml of 24,25-(OH)2-D). A seasonal variation was demonstrated in serum levels of 24,25-(OH)2-D as well as those of 25-OH-D. Serum levels of 24,25-(OH)2-D were highly correlated (gamma=0.884, p<0.001) with those of 25-OH-D in normal human subjects, and the percentage ratio of 24,25-(OH)2-D/25-OH-D was 7.4 +/- 1.4% (mean +/- SD), irrespective of age or seasonal variations.
采用竞争性蛋白结合分析法，对1ml人血清脂质提取物中的25-羟基维生素D（25-OH-D）和24,25-二羟基维生素D [24,25-(OH)2-D]血清浓度进行了测定。通过葡聚糖凝胶LH-20柱色谱法从脂质提取物中分离出25-OH-D和24,25-(OH)2-D。在测定之前，通过高压液相色谱法对葡聚糖凝胶LH-20柱上的24,25-(OH)2-D馏分进行进一步纯化。在各年龄组中，正常男性的25-OH-D和24,25-(OH)2-D血清水平与正常女性相似。新生儿的25-OH-D和24,25-(OH)2-D血清水平分别为8.28±1.68 ng/ml（均值±标准差）和0.55±0.16 ng/ml，显著低于成年人（25-OH-D为21.3±4.8 ng/ml，24,25-(OH)2-D为1.55±0.31 ng/ml，p<0.001）。24,25-(OH)2-D以及25-OH-D的血清水平均呈现季节性变化。在正常人体中，24,25-(OH)2-D的血清水平与25-OH-D的血清水平高度相关（γ=0.884，p<0.001），且24,25-(OH)2-D/25-OH-D的百分比比值为7.4±1.4%（均值±标准差），与年龄或季节变化无关。
