Lawman M J, Courtney R J, Eberle R, Schaffer P A, O'Hara M K, Rouse B T
Infect Immun. 1980 Nov;30(2):451-61. doi: 10.1128/iai.30.2.451-461.1980.
This communication deals with the question of which of the viral antigens constitutes the targets for cytotoxic T lymphocytes (CTL) generated against herpes simplex virus type 1 (HSV-1). The approach used was, first, to compare cytotoxicity of CTL against target cells infected with virus in the presence of tunicamycin and 2-deoxy-D-glucose, which are known to inhibit glycoprotein synthesis, and second, to compare cytotoxicity of CTL against target cells infected with wild-type HSV-1 with that against target cells infected with a temperature-sensitive mutant of HSV-1 which, at the nonpermissive temperature, exhibits diminished glycoprotein synthesis. The results show that glycoprotein expression is required for the demonstration of cytotoxic activity of CTL. The level of cytotoxicity against the temperature-sensitive HSV-1 target at the nonpermissive temperature was reduced and correlated with the level of expression of the major envelope glycoprotein region (VP123; molecular weight = 123,000) at the target cell surface as measured serologically by antibody binding studies. The results were interpreted to indicate that HSV-1-induced glycoproteins are the target antigens for anti-HSV CTL and that the principal viral antigens recognized by the CTL may be glycoproteins of the VP123 region.
本通讯探讨了针对单纯疱疹病毒1型（HSV-1）产生的细胞毒性T淋巴细胞（CTL）的靶标是哪种病毒抗原的问题。所采用的方法，首先是比较在衣霉素和2-脱氧-D-葡萄糖存在的情况下，CTL对感染病毒的靶细胞的细胞毒性，已知这两种物质可抑制糖蛋白合成；其次是比较CTL对感染野生型HSV-1的靶细胞的细胞毒性与对感染HSV-1温度敏感突变体的靶细胞的细胞毒性，该突变体在非允许温度下糖蛋白合成减少。结果表明，糖蛋白表达是CTL细胞毒性活性表现所必需的。在非允许温度下，针对温度敏感型HSV-1靶标的细胞毒性水平降低，并且与通过抗体结合研究血清学测定的靶细胞表面主要包膜糖蛋白区域（VP123；分子量 = 123,０００）的表达水平相关。这些结果被解释为表明HSV-1诱导的糖蛋白是抗HSV CTL的靶抗原，并且CTL识别的主要病毒抗原可能是VP123区域的糖蛋白。
