Grishina T I, Alekseeva O G
J Immunol Methods. 1980;38(3-4):309-13. doi: 10.1016/0022-1759(80)90279-3.
A technically simple procedure is described for simultaneous determination of numbers and functions of human blood lymphocyte subpopulations. The method is based on stimulation with mitogens (PHA, Con A and PWM) with simultaneous rosetting to detect E receptors and C3 receptors. Con A and PWM activate the affinity of C3 receptors in normal lymphocytes increasing the number of cells rosetting with zymosan-complement particles, and also the number of cells giving rosettes with both zymosan-complement particles and sheep red blood cells (B cells and 'D' cells respectively). PHA activates E receptors, increasing the number of T cells detected. The lymphocytes of 33 patients with allergic diseases of the skin and respiratory tract showed a decrease in C3 rosettes during stimulation with Con A and PWM, which suggests a defect of helper and suppressor functions of lymphocytes in these patients.
本文描述了一种技术上简单的程序，用于同时测定人血淋巴细胞亚群的数量和功能。该方法基于用促细胞分裂剂（PHA、Con A和PWM）刺激，同时进行玫瑰花结试验以检测E受体和C3受体。Con A和PWM激活正常淋巴细胞中C3受体的亲和力，增加与酵母多糖 - 补体颗粒形成玫瑰花结的细胞数量，以及与酵母多糖 - 补体颗粒和绵羊红细胞都形成玫瑰花结的细胞数量（分别为B细胞和“D”细胞）。PHA激活E受体，增加检测到的T细胞数量。33例皮肤和呼吸道过敏性疾病患者的淋巴细胞在Con A和PWM刺激期间显示C3玫瑰花结减少，这表明这些患者淋巴细胞的辅助和抑制功能存在缺陷。
