Cheredeev A N, Golovistikov I N, Semenkov V F, Pavliuk A S, Chernysheva I P
Zh Mikrobiol Epidemiol Immunobiol. 1980 Jun(6):67-71.
Antisera to human B-lymphocytes were obtained by immunization of rabbits with lymphocytes obtained from peripheral blood taken from healthy persons and patients having chronic lympholeukosis with B-cell proliferation. These antisera were subjected to adsorption on human erythrocytes, liver and thymocytes. The specificity of B-lymphocyte antiserum was evaluated in the E- and EAC-rosette-formation inhibition test and by the comparison of the number of lymphocytes revealed in the cytotoxic test with EAC-rosette-forming cells. Besides the above-mentioned tests, the specificity of rabbit antiserum to human thymocyte membranes was also determined in the test of inhibiting T-lymphocyte stimulation with phytohemagglutinin. The antisera thus obtained were specific, in definite dilutions, to human T- and B-lymphocytes.
用人外周血淋巴细胞和慢性淋巴细胞白血病（B细胞增殖型）患者的淋巴细胞免疫家兔，获得了抗人B淋巴细胞抗血清。这些抗血清用人类红细胞、肝脏和胸腺细胞进行吸附处理。通过E和EAC玫瑰花结形成抑制试验以及比较细胞毒性试验中EAC玫瑰花结形成细胞与所检测到的淋巴细胞数量，评估了B淋巴细胞抗血清的特异性。除上述试验外，还用植物血凝素抑制T淋巴细胞刺激试验测定了兔抗人胸腺细胞膜抗血清的特异性。如此获得的抗血清在特定稀释度下对人T淋巴细胞和B淋巴细胞具有特异性。
