[Methodologic features of the production of heterologous antisera to human T- and B-lymphocytes and evaluation of their specificity].

Antisera to human B-lymphocytes were obtained by immunization of rabbits with lymphocytes obtained from peripheral blood taken from healthy persons and patients having chronic lympholeukosis with B-cell proliferation. These antisera were subjected to adsorption on human erythrocytes, liver and thymocytes. The specificity of B-lymphocyte antiserum was evaluated in the E- and EAC-rosette-formation inhibition test and by the comparison of the number of lymphocytes revealed in the cytotoxic test with EAC-rosette-forming cells. Besides the above-mentioned tests, the specificity of rabbit antiserum to human thymocyte membranes was also determined in the test of inhibiting T-lymphocyte stimulation with phytohemagglutinin. The antisera thus obtained were specific, in definite dilutions, to human T- and B-lymphocytes.