Morita T, Morita R, Takagi S, Shimoyama N, Nagai K
Tohoku J Exp Med. 1981 Mar;133(3):275-84. doi: 10.1620/tjem.133.275.
Antisera were prepared in rabbits by immunization with either human peripheral blood T lymphocytes (PTL) or tonsil B lymphocytes (TBL). Specificities of sequentially absorbed antisera were examined by cytotoxicity testing, immunoperoxidase staining and absorption study. Anti-PTL serum (ATS) reacted with E rosetting lymphocytes in the normal population. ATS was also reactive with some of lymphoid neoplasms, whether neoplastic cells carried E receptors or not. This serum did not lyse any of myelogenous leukemia cells. When antiserum was used in the immunoperoxidase staining, ATS strongly stained paracortical cells in the lymph node and medullary thymocytes in the thymus. On the other hand, anti-TBL serum (ABS) was reactive with lymphocytes corresponding to the percentage of EAC binding lymphocytes in the normal population. ABS reacted not only with the neoplastic cells carrying C3 receptors and/or surface immunoglobulin, but also with non T . non B-acute lymphoblastic leukemia cells and a small number of acute myelogenous leukemia cells. In the immunoperoxidase staining, lymph follicular cells showed positive immunoreactivity with ABS, but paracortical cells did not.
通过用人外周血T淋巴细胞（PTL）或扁桃体B淋巴细胞（TBL）免疫兔子来制备抗血清。通过细胞毒性试验、免疫过氧化物酶染色和吸收研究来检测顺序吸收抗血清的特异性。抗PTL血清（ATS）与正常人群中的E花环形成淋巴细胞发生反应。ATS也与一些淋巴样肿瘤发生反应，无论肿瘤细胞是否携带E受体。该血清不能溶解任何髓性白血病细胞。当抗血清用于免疫过氧化物酶染色时，ATS强烈染色淋巴结中的副皮质细胞和胸腺中的髓质胸腺细胞。另一方面，抗TBL血清（ABS）与正常人群中与EAC结合淋巴细胞百分比相对应的淋巴细胞发生反应。ABS不仅与携带C3受体和/或表面免疫球蛋白的肿瘤细胞发生反应，还与非T、非B急性淋巴细胞白血病细胞和少数急性髓性白血病细胞发生反应。在免疫过氧化物酶染色中，淋巴滤泡细胞对ABS呈阳性免疫反应，但副皮质细胞则没有。
