Moriuchi T, Kasai M, Yamaguchi H, Kobayashi H
Cancer Res. 1981 Feb;41(2):708-12.
Antisera to the 1-butyl-1-nitrosourea-induced "non-T, non-B" rat leukemia line DBLA-6 were raised in rabbits. Following absorption with syngeneic hepatoma cells, the antisera were very similar in specificity to antisera raised to rat Thy-1 antigen. Anti-DBLA-6 serum was cytotoxic in the presence of complement against 70 to 90% of thymocytes and 40 to 50% of neonatal spleen cells. In contrast, no significant cytotoxicity was observed against cells from bone marrow, lymph node, spleen, and peritoneum. An absorption test revealed that an antigen recognized by anti-DBLA-6 serum was present in brain tissue but absent in liver and kidney tissues. Nineteen rat leukemias and lymphomas were divided into six groups based on antigenic and morphological characteristics and the presence of receptor for guinea pig red blood cells. These tumors were investigated for the presence of the antigen recognized by anti-DBLA-6 serum. Of the leukemias and lymphomas studied, anti-DBLA-6 serum reacted with all thymic (Group 1) and extrathymic (Group 2) lymphomas and unclassified leukemias (Groups 3 and 4), while all myelogenous leukemias (Group 5) and erythroleukemias (Group 6) were negative. The position of leukemias and lymphomas reactive with anti-DBLA-6 serum in the lymphocyte maturational pathway is discussed.
用1-丁基-1-亚硝基脲诱导的“非T、非B”大鼠白血病细胞系DBLA-6制备兔抗血清。用同基因肝癌细胞吸收后，这些抗血清的特异性与针对大鼠Thy-1抗原制备的抗血清非常相似。抗DBLA-6血清在补体存在下对70%至90%的胸腺细胞和40%至50%的新生脾细胞具有细胞毒性。相比之下，对来自骨髓、淋巴结、脾脏和腹膜的细胞未观察到明显的细胞毒性。吸收试验表明，抗DBLA-6血清识别的一种抗原存在于脑组织中，而不存在于肝脏和肾脏组织中。根据抗原性、形态学特征以及豚鼠红细胞受体的存在情况，将19种大鼠白血病和淋巴瘤分为6组。研究了这些肿瘤中抗DBLA-6血清识别的抗原的存在情况。在所研究的白血病和淋巴瘤中，抗DBLA-6血清与所有胸腺（第1组）和胸腺外（第2组）淋巴瘤以及未分类的白血病（第3组和第4组）发生反应，而所有髓性白血病（第5组）和红白血病（第6组）均为阴性。讨论了与抗DBLA-6血清反应的白血病和淋巴瘤在淋巴细胞成熟途径中的位置。
