Heldin C H, Wasteson A, Westermark B
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6611-5. doi: 10.1073/pnas.77.11.6611.
DNA synthesis and cell division were measured in serum-free cultures of human normal diploid glial cells maintained in MCDB 105 medium. In growth factor-free cultures the cells remained viable but the cell number was essentially constant. Supplementation with 10 ng of epidermal growth factor or platelet-derived growth factor per ml significantly stimulated DNA synthesis and cell multiplication. Growth occurred both when cells were allowed to settle in serum-containing medium and when cells were plated in serum-free medium. In the latter type of cultures, the cell yield was improved bu incubating the cells in collagen-coated dishes. The use of a miniclone technique allowed the analysis of cell multiplication induced by platelet-derived growth factor at the clonal level and demonstrated that the growth factor induced several cell cycle rounds in a large fraction of clones. The results show that normal cells grown in a recently developed synthetic medium (MCDB 105) supplemented with pure growth factors may multiply without the addition of plasma-derived factors ("progression factors"). It is suggested that the need fo progression factors may simply depend on the composition of the synthetic nutrient medium.
在MCDB 105培养基中培养的人正常二倍体神经胶质细胞的无血清培养物中，对DNA合成和细胞分裂进行了检测。在无生长因子的培养物中，细胞仍保持活力，但细胞数量基本保持恒定。每毫升添加10纳克表皮生长因子或血小板衍生生长因子可显著刺激DNA合成和细胞增殖。当细胞接种于含血清培养基中以及接种于无血清培养基中时，细胞均可生长。在后一种培养类型中，通过将细胞培养在胶原包被的培养皿中可提高细胞产量。使用微型克隆技术可在克隆水平分析血小板衍生生长因子诱导的细胞增殖，并证明该生长因子可在大部分克隆中诱导多个细胞周期循环。结果表明，在添加了纯生长因子的最新开发的合成培养基（MCDB 105）中生长的正常细胞，无需添加血浆衍生因子（“进展因子”）即可增殖。有人提出，对进展因子的需求可能仅取决于合成营养培养基的组成。
