Hoessli D C, Vassalli P, Pink J R
Eur J Immunol. 1980 Nov;10(11):814-21. doi: 10.1002/eji.1830101104.
Two-dimensional electrophoresis was used to analyze the surface glycoproteins of murine thymocytes and lymph node cells. Two-dimensional maps of unselected, radioiodinated lymphocyte surface proteins were complex, showing at least 20 different components, but simpler patterns were obtained by using rabbit antibodies directed against the surface proteins of a T lymphoma cell line, to precipitate xenoantigens from lysates of radioiodinated or biosynthetically labeled thymocytes and lymph node cells. These xenoantibodies precipitated 12-13 distinct components from each cell type, of which all but 3 were sialoglycoproteins. Two types of difference between the surface glycoproteins of thymocytes and peripheral lymphocytes could be detected. First, higher mol. wt. glycoproteins and Thy-1 are more acidic in peripheral lymphocytes than in thymocytes, and this difference disappears after neuraminidase treatment. One additional high mol. wt. glycoprotein is also detectable in peripheral lymphocytes, probably reflecting the greater carbohydrate complexity of these molecules, when expressed on such cells. Second, 3 glycoproteins are strongly labeled only on thymocytes, and 3 others only on peripheral lymphocytes. These 6 glycoproteins might represent genuine differentiation antigens.
二维电泳用于分析小鼠胸腺细胞和淋巴结细胞的表面糖蛋白。未选择的、经放射性碘化的淋巴细胞表面蛋白的二维图谱很复杂，显示至少有20种不同的成分，但通过使用针对T淋巴瘤细胞系表面蛋白的兔抗体，从放射性碘化或生物合成标记的胸腺细胞和淋巴结细胞裂解物中沉淀异种抗原，可得到更简单的图谱。这些异种抗体从每种细胞类型中沉淀出12 - 13种不同的成分，其中除3种外均为唾液酸糖蛋白。可检测到胸腺细胞和外周淋巴细胞表面糖蛋白之间的两种差异。第一，外周淋巴细胞中较高分子量的糖蛋白和Thy-1比胸腺细胞中的更具酸性，经神经氨酸酶处理后这种差异消失。在外周淋巴细胞中还可检测到一种额外的高分子量糖蛋白，这可能反映了这些分子在这类细胞上表达时碳水化合物的复杂性更高。第二，3种糖蛋白仅在胸腺细胞上被强烈标记，另外3种仅在外周淋巴细胞上被强烈标记。这6种糖蛋白可能代表真正的分化抗原。
