Thiel E
Fortschr Med. 1980 Nov 27;98(44):1736-41.
A quantitative autoradiographic method is presented for determining absolute amounts of 125I-labelled compounds on the surface of individual cells. Autoradiographic evaluation of single cell radioactivity is accomplished by comparing the silver grain densities over the specimen and a radioactive standard being exposed simultaneously. The application of microphotometry of grain densities permits to determine semi-automatically the content of surface antigens or receptors of single cells in immunoautoradiographs. Hereby, the expression of various surface markers was measured on single lymphocytes and leukaemia cells. It was demonstrated that the variation of surface immunoglobulins or T cell antigens is restricted in density in the situation of leukaemia. This fits the concept that leukaemic cell clones are arrested at certain stages of cell differentiation. An immunological classification of acute and chronic forms of lymphatic leukaemias is given in relation to the differentiation degree of the neoplastic cell.
本文介绍了一种定量放射自显影方法，用于测定单个细胞表面125I标记化合物的绝对量。通过比较标本上的银粒密度与同时曝光的放射性标准品来完成单细胞放射性的放射自显影评估。利用银粒密度的显微光度测定法可在免疫放射自显影中半自动地测定单个细胞表面抗原或受体的含量。据此，在单个淋巴细胞和白血病细胞上测量了各种表面标志物的表达。结果表明，在白血病情况下，表面免疫球蛋白或T细胞抗原的密度变化受到限制。这符合白血病细胞克隆在细胞分化的某些阶段停滞的概念。根据肿瘤细胞的分化程度对急性和慢性淋巴细胞白血病进行了免疫学分类。
