Sawada J, Osawa T
Immunology. 1980 Nov;41(3):525-34.
The cellular cytotoxicity mediated by mouse spleen cells in the presence of mitogenic or non-mitogenic lectins was established under serum-free conditions and characterized. Compared with the lectin-induced cellular cytotoxicity (LICC) in the guinea-pig, the activity of lymphotoxin (LT) released into the murine LICC assay cultures was very low. However, a positive correlation was found between the strength of LICC and the LT activity released into the supernatants. Moreover, the addition of puromycin, a potent enhancing reagent of guinea-pig LT activity, markedly promoted the LICC when added 4 h after the initiation of the LICC culture. These data, taken together, suggest that LT acts as an effector molecule in the murine LICC systems as well as in the guinea-pig LICC systems. Properties of the effector cell populations mediating LICC were investigated by depletion of plastic-adherent or nylon-wool adherent cells, by treatment of spleen cells with anti-T-cell sera and complement, and by use of nude mouse spleen cells. The results obtained suggest that both concanavalin A and phytohaemagglutinin-P can induce nylon-wool non-adherent T-cell mediated LICC, phytohaemagglutinin-W was found to be capable of inducing both the nylon-wool non-adherent T-cell mediated LICC and the nylon-wool adherent non-T-cell mediated LICC, the major effector of Bauhinia purpurea agglutinin-LICC was found to be the nylon-adherent non-T-cell population.
在无血清条件下建立并表征了小鼠脾细胞在有丝分裂原或非有丝分裂原凝集素存在下介导的细胞毒性。与豚鼠中凝集素诱导的细胞毒性（LICC）相比，在小鼠LICC测定培养物中释放的淋巴毒素（LT）活性非常低。然而，发现LICC的强度与释放到上清液中的LT活性之间存在正相关。此外，添加嘌呤霉素（一种豚鼠LT活性的有效增强剂），在LICC培养开始4小时后添加时，显著促进了LICC。综合这些数据表明，LT在小鼠LICC系统以及豚鼠LICC系统中均作为效应分子起作用。通过去除塑料贴壁或尼龙毛贴壁细胞、用抗T细胞血清和补体处理脾细胞以及使用裸鼠脾细胞，研究了介导LICC的效应细胞群体的特性。获得的结果表明，刀豆球蛋白A和植物血凝素-P均可诱导尼龙毛非贴壁T细胞介导的LICC，发现植物血凝素-W能够诱导尼龙毛非贴壁T细胞介导的LICC和尼龙毛贴壁非T细胞介导的LICC，发现紫羊蹄甲凝集素-LICC的主要效应细胞是尼龙贴壁非T细胞群体。
