Ferrante A, Smyth C
Immunology. 1984 Mar;51(3):461-8.
The whole-killed pathogenic free-living amoeba, Naegleria fowleri, contained mitogenic activity (NFM) for mouse spleen cells. Similar preparations from the non-pathogenic amoeba N. gruberi and the pathogenic Acanthamoeba culbertsoni lacked mitogenic activity. Fluids from N. fowleri cultures, containing amoeba antigens, also failed to cause proliferation of mouse spleen lymphocytes. Spleen cells from athymic nude mice failed to respond to NFM. In addition, nylon wool non-adherent, but not the adherent, spleen cell subpopulation proliferated in the presence of NFM. These results show that the factor(s) is mitogenic for T lymphocytes. The spleen cells from mice treated with cyclophosphamide doses known to deplete T suppressor cell activity from this organ failed to respond to NFM, indicating that NFM may be mitogenic for T suppressor cells.
全灭活的致病性自由生活阿米巴——福氏耐格里阿米巴，对小鼠脾细胞具有促有丝分裂活性（NFM）。来自非致病性阿米巴——格氏耐格里阿米巴以及致病性棘阿米巴的类似制剂则缺乏促有丝分裂活性。含有阿米巴抗原的福氏耐格里阿米巴培养液也未能引起小鼠脾淋巴细胞增殖。无胸腺裸鼠的脾细胞对NFM无反应。此外，在NFM存在的情况下，尼龙毛非黏附性脾细胞亚群（而非黏附性亚群）发生增殖。这些结果表明，该因子对T淋巴细胞具有促有丝分裂作用。用已知可耗尽该器官中T抑制细胞活性的环磷酰胺剂量处理的小鼠脾细胞对NFM无反应，这表明NFM可能对T抑制细胞具有促有丝分裂作用。
