Anzano M A, Lamb A J, Olson J A
J Nutr. 1981 Mar;111(3):496-504. doi: 10.1093/jn/111.3.496.
Rapid and essentially synchronous vitamin A deficiency was induced by the withdrawal of retinoic acid from stringently deficient animals reared by feeding early weight plateau vitamin A-deprived male rats diets first supplemented with and then lacking in 2 micrograms retinoic acid/g diet in repeating 18:10 day cycles. Secondary inanition was minimized by the control led force-feeding of deficient and control animals. The time to inset of pilocarpine (3 mg/kg body weight) induced salivation increased progressively starting 6-8 days after retionate withdrawal. Concomitantly, saliva volumes in the 20 minutes following the onset of salivation decreased. Protein and alpha-amylase concentrations were constant until around days 10-12 (T10-12) of deficiency but then decreased. Synthesis of proteins was normal, however as judged by total parotid gland alpha-amylase activity (T14). Seemingly, vitamin A deficiency may directly affect cells involved in saliva secretion, since decreased secretory function was noted several days prior to keratinization and blockage of the striated and excretory ducts.
通过在严格缺乏维生素A的动物中撤去视黄酸来诱导快速且基本同步的维生素A缺乏，这些动物是通过给早期体重稳定的维生素A缺乏雄性大鼠喂食首先添加然后缺乏每克饮食2微克视黄酸的日粮，以重复18:10天的周期饲养的。通过对缺乏和对照动物进行受控强制喂食，将继发性营养不良降至最低。从撤去视黄酸后6 - 8天开始，毛果芸香碱（3毫克/千克体重）诱导流涎的时间逐渐增加。同时，流涎开始后20分钟内的唾液量减少。蛋白质和α-淀粉酶浓度在缺乏的第10 - 12天（T10 - 12）左右之前保持恒定，但随后下降。然而，根据腮腺总α-淀粉酶活性（T14）判断，蛋白质合成正常。似乎维生素A缺乏可能直接影响参与唾液分泌的细胞，因为在角质化以及纹状管和排泄管阻塞之前几天就观察到分泌功能下降。
