Rachkov S M, Bekbergenov B M, Korolev P N, Glezer G A
Antibiotiki. 1981 Jan;26(1):55-8.
The effect of some antibiotics on in vitro blast transformation of lymphocytes of healthy donors stimulated with phytohemagglutinin was studied. The stimulation level was estimated by incorporation of 3H-thymidine into lymphocytes. It was shown that minocycline had a markedly pronounced inhibitory activity. Chloramphenicol and tetracycline also proved to be active inhibitors. Penicillin, cephalotin and carbenicillin had no effect on lymphocyte mitogenesis. Gentamicin proved to be a rather active stimulator of mitogenesis, especially with the use of a suboptimal concentration of phytohemagglutinin. The method is rather sensitive and informative. It may be used for preliminary determination of the immunotropic properties of drugs and chemical compounds with respect to immunocompetent cells of man.
研究了某些抗生素对健康供体经植物血凝素刺激的淋巴细胞体外增殖转化的影响。通过将3H-胸腺嘧啶核苷掺入淋巴细胞来评估刺激水平。结果表明，米诺环素具有明显的抑制活性。氯霉素和四环素也被证明是活性抑制剂。青霉素、头孢噻吩和羧苄青霉素对淋巴细胞有丝分裂没有影响。庆大霉素被证明是一种相当活跃的有丝分裂刺激剂，尤其是在使用次优浓度的植物血凝素时。该方法相当灵敏且信息丰富。它可用于初步测定药物和化合物对人体免疫活性细胞的免疫调节特性。
