人淋巴细胞亚群中性糖鞘脂的定量分析。
Quantitative analysis of neutral glycosphingolipids from human lymphocyte subpopulations.
作者信息
Schwarting G A
出版信息
Biochem J. 1980 Sep 1;189(3):407-12. doi: 10.1042/bj1890407.
Abstract
The glycosphingolipids of normal human lymphocytes from individual donors were analysed by high-pressure liquid chromatography. In addition, purified T- and B-lymphocytes were examined separately. Lactosylceramide was shown to be the major neutral glycosphingolipid in human lymphocytes, and monohexosylceramide, trihexosylceramide, globoside and paragloboside were all detected in smaller amounts. Analysis of purified B- and T-cell fractions revealed that each of these populations contained a similar qualitative profile for neutral glycosphingolipids, but that quantitatively, B-cells contained several times more of each glycosphingolipid per cell than did T-cells.
摘要
采用高压液相色谱法分析了来自个体供体的正常人淋巴细胞的糖鞘脂。此外，还分别对纯化的T淋巴细胞和B淋巴细胞进行了检测。结果表明，乳糖基神经酰胺是人类淋巴细胞中的主要中性糖鞘脂，同时还检测到少量的单己糖神经酰胺、三己糖神经酰胺、红细胞糖苷脂和异红细胞糖苷脂。对纯化的B细胞和T细胞组分的分析显示，这些细胞群体中的每一种中性糖鞘脂在质量上具有相似的分布，但在数量上，每个B细胞所含的每种糖鞘脂比T细胞多几倍。
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