Mukhin D N, Prokazova N V
Institute of Experimental Cardiology, National Cardiology Research Centre, Moscow, Russia.
Atherosclerosis. 1992 Apr;93(3):173-7. doi: 10.1016/0021-9150(92)90253-d.
We have investigated the content and composition of neutral glycosphingolipids (GSLs) in the cells isolated by enzyme digestion from elastic-hyperplastic and musculo-elastic intimal layers of grossly normal and atherosclerotic regions of human aorta. We have detected three types of neutral GSLs in the intimal cells identified as glucosylceramide, trihexosylceramide and tetrahexosylceramide. We failed to detect lactosylceramide in the intimal cells. The cells of the elastic-hyperplastic layer of grossly normal regions contained trihexosylceramide and tetrahexosylceramide, while glucosylceramide was not detected. Considerable amounts of glucosylceramide were found, and the trihexosylceramide and tetrahexosylceramide content was increased in the cells isolated from atherosclerotic regions. The cells of the musculo-elastic layer of grossly normal intimal regions contained glucosylceramide, trihexosylceramide and tetrahexosylceramide. Cells of the musculo-elastic layer of the fatty streak contained noticeable higher amounts of glucosylceramide, as well as greater amounts of trihexosylceramide and tetrahexosylceramide. Cells of the musculo-elastic layer of the plaque also appeared to contain more glucosylceramide, tetrahexosylceramide, but less trihexosylceramide as compared with grossly normal regions. In both cases cells of the fatty streak exhibited the highest total amount of neutral GSLs, but at the same time the neutral GSL composition of the fatty streak was not similar to GSL composition which is known for human blood monocytes. These findings indicate that elevation of neutral GSL level is observed in cells from atherosclerotic lesions of human aortic intima.
我们研究了从人主动脉大体正常区域和动脉粥样硬化区域的弹性增生内膜层和肌弹性内膜层通过酶消化分离出的细胞中中性糖鞘脂（GSLs）的含量和组成。我们在内膜细胞中检测到三种类型的中性GSLs，分别鉴定为葡糖神经酰胺、三己糖神经酰胺和四己糖神经酰胺。我们在内膜细胞中未检测到乳糖神经酰胺。大体正常区域弹性增生层的细胞含有三己糖神经酰胺和四己糖神经酰胺，但未检测到葡糖神经酰胺。在从动脉粥样硬化区域分离出的细胞中发现了大量的葡糖神经酰胺，且三己糖神经酰胺和四己糖神经酰胺的含量增加。大体正常内膜区域肌弹性层的细胞含有葡糖神经酰胺、三己糖神经酰胺和四己糖神经酰胺。脂肪条纹肌弹性层的细胞含有明显更高含量的葡糖神经酰胺，以及更多的三己糖神经酰胺和四己糖神经酰胺。与大体正常区域相比，斑块肌弹性层的细胞似乎也含有更多的葡糖神经酰胺、四己糖神经酰胺，但三己糖神经酰胺较少。在这两种情况下，脂肪条纹的细胞中性GSLs总量最高，但同时脂肪条纹的中性GSL组成与已知的人血单核细胞的GSL组成不同。这些发现表明，在人主动脉内膜动脉粥样硬化病变的细胞中观察到中性GSL水平升高。
