吲哚胺2,3-双加氧酶。一种新的、快速、灵敏的放射性测定法及其在大鼠组织中该酶研究中的应用。
Indoleamine 2,3-dioxygenase. A new, rapid, sensitive radiometric assay and its application to the study of the enzyme in rat tissues.
作者信息
Cook J S, Pogson C I, Smith S A
出版信息
Biochem J. 1980 Sep 1;189(3):461-6. doi: 10.1042/bj1890461.
Abstract
A simple and convenient assay for indoleamine 2,3-dioxygenase has been developed. This depends on the conversion of D-[ring-2-14C]tryptophan to [14C]formate, excess substrate is removed by adsorption onto charcoal. This assay, which is 20-fold more sensitive than previous procedures, is applicable both to crude extracts and to large numbers of samples. Activity in rat tissues is very much lower than in those of the rabbit; measureable activity is found only in the stomach, spleen, intestine and kidney. Enzyme activity in the rat intestine was increased by 50% in rats pretreated with L-tryptophan.
摘要
已开发出一种简单便捷的吲哚胺2,3-双加氧酶检测方法。该方法基于将D-[环-2-¹⁴C]色氨酸转化为[¹⁴C]甲酸，通过吸附到活性炭上去除过量底物。此检测方法比以前的方法灵敏20倍，适用于粗提物和大量样本。大鼠组织中的活性远低于兔子；仅在胃、脾、肠和肾中发现可测量的活性。用L-色氨酸预处理的大鼠，其肠中的酶活性增加了50%。
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Indoleamine 2,3-dioxygenase. A new, rapid, sensitive radiometric assay and its application to the study of the enzyme in rat tissues.吲哚胺2,3-双加氧酶。一种新的、快速、灵敏的放射性测定法及其在大鼠组织中该酶研究中的应用。
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The activity of phosphoenolpyruvate carboxykinase in rat tissues. Assay techniques and effects of dietary and hormonal changes.大鼠组织中磷酸烯醇式丙酮酸羧激酶的活性。测定技术以及饮食和激素变化的影响。
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