大鼠组织中磷酸烯醇式丙酮酸羧激酶的活性。测定技术以及饮食和激素变化的影响。
The activity of phosphoenolpyruvate carboxykinase in rat tissues. Assay techniques and effects of dietary and hormonal changes.
作者信息
Pogson C I, Smith S A
出版信息
Biochem J. 1975 Nov;152(2):401-8. doi: 10.1042/bj1520401.
Abstract
- Phosphoenolpyruvate carboxykinase was assayed by three methods: (i) incorporation of H(14)CO(3) (-) into oxaloacetate: (ii) conversion of oxaloacetate into phosphoenolpyruvate, subsequently assayed enzymically; and (iii) transfer of (32)P from [gamma-(32)P]GTP to oxaloacetate. 2. Enzyme activity is increased in liver and epididymal adipose tissue in alloxan-diabetes and starvation, and in kidney in starved, acidotic and steroid-treated animals. 3. The ratios of the ;back' to the ;forward' reactions in liver, kidney and epididymal adipose tissue are different and characteristic of each tissue; they differ markedly from values reported for the purified mitochondrial enzyme. 4. The ratio of the ;back' to ;forward' reaction in any one tissue is constant in adrenalectomized, diabetic, acidotic and steroid-treated animals. 5. In starved animals, the ratio is increased in liver and kidney, but decreased in epididymal adipose tissue. 6. Administration of l-tryptophan results in an acute (1h) increase in activity measured in the ;forward' direction alone in liver and epididymal adipose tissue, but not in kidney.
摘要
- 磷酸烯醇丙酮酸羧激酶通过三种方法进行测定：（i）将H（14）CO（3）（-）掺入草酰乙酸；（ii）将草酰乙酸转化为磷酸烯醇丙酮酸，随后进行酶法测定；（iii）将[γ-（32）P]GTP中的（32）P转移至草酰乙酸。2. 在四氧嘧啶糖尿病和饥饿状态下，肝脏和附睾脂肪组织中的酶活性增加，在饥饿、酸中毒和接受类固醇治疗的动物的肾脏中酶活性也增加。3. 肝脏、肾脏和附睾脂肪组织中“逆向”反应与“正向”反应的比率各不相同，且具有各组织的特征；它们与纯化的线粒体酶所报道的值显著不同。4. 在肾上腺切除、糖尿病、酸中毒和接受类固醇治疗的动物中，任何一个组织中“逆向”反应与“正向”反应的比率是恒定的。5. 在饥饿的动物中，肝脏和肾脏中的比率增加，但附睾脂肪组织中的比率降低。6. 给予l-色氨酸会导致仅在肝脏和附睾脂肪组织中测量的“正向”方向的活性急性（1小时）增加，而在肾脏中则不会。
相似文献
1
The activity of phosphoenolpyruvate carboxykinase in rat tissues. Assay techniques and effects of dietary and hormonal changes.大鼠组织中磷酸烯醇式丙酮酸羧激酶的活性。测定技术以及饮食和激素变化的影响。
Biochem J. 1975 Nov;152(2):401-8. doi: 10.1042/bj1520401.
2
Regulation of phosphoenolpyruvate carboxykinase (GTP) in adipose tissue in vivo by glucocorticoids and insulin.糖皮质激素和胰岛素对体内脂肪组织中磷酸烯醇式丙酮酸羧激酶（GTP）的调节作用。
Biochem J. 1976 Jul 15;158(1):1-7. doi: 10.1042/bj1580001.
3
4
The effect of steroids and ammonium chloride acidosis on phosphoenolpyruvate carboxykinase in rat kidney cortex. II. The kinetics of enzyme induction.类固醇和氯化铵酸中毒对大鼠肾皮质磷酸烯醇丙酮酸羧激酶的影响。II. 酶诱导动力学。
J Clin Invest. 1972 Sep;51(9):2284-91. doi: 10.1172/JCI107038.
5
Effect of cold exposure on phosphoenolpyruvate carboxykinase (GTP) activity and cyclic amp concentration in livers of starved rats. Role of glucorticoids.冷暴露对饥饿大鼠肝脏中磷酸烯醇丙酮酸羧激酶（GTP）活性和环磷酸腺苷浓度的影响。糖皮质激素的作用。
Biochim Biophys Acta. 1976 Oct 22;444(3):694-703. doi: 10.1016/0304-4165(76)90316-0.
6
7
The concentration of P-enolpyruvate carboxykinase protein in murine tissues in diabetes of chemical and genetic origin.化学性和遗传性糖尿病小鼠组织中磷酸烯醇式丙酮酸羧激酶蛋白的浓度
J Biol Chem. 1983 Dec 10;258(23):14257-62.
8
Glucocorticoids and the regulation of phosphoenolpyruvate carboxykinase (guanosine triphosphate) in the rat.糖皮质激素与大鼠磷酸烯醇式丙酮酸羧激酶（三磷酸鸟苷）的调节
Biochem J. 1975 Aug;150(2):195-203. doi: 10.1042/bj1500195.
9
Glucocorticoid receptors and regulation of phosphoenolpyruvate carboxykinase activity in rat kidney and adipose tissue.糖皮质激素受体与大鼠肾脏和脂肪组织中磷酸烯醇式丙酮酸羧激酶活性的调节
Am J Physiol. 1977 Sep;233(3):E147-51. doi: 10.1152/ajpendo.1977.233.3.E147.
10
The effects of starvation and experimental diabetes on phosphoenol-pyruvate carboxykinase in the guinea pig.饥饿和实验性糖尿病对豚鼠磷酸烯醇丙酮酸羧激酶的影响。
Biochem J. 1977 May 15;164(2):357-61. doi: 10.1042/bj1640357.
引用本文的文献
1
Transfer of dysbiotic gut microbiota has beneficial effects on host liver metabolism.肠道微生物群失调的转移对宿主肝脏代谢具有有益影响。
Mol Syst Biol. 2017 Mar 16;13(3):921. doi: 10.15252/msb.20167356.
2
Metabolic effects of feeding a high protein/low carbohydrate diet as compared to a low protein/high carbohydrate diet to rainbow troutSalmo gairdneri.与低蛋白/高碳水化合物饮食相比，高蛋白/低碳水化合物饮食对虹鳟鱼（Salmo gairdneri）的代谢影响。
Fish Physiol Biochem. 1986 Jan;1(1):7-15. doi: 10.1007/BF02309589.
3
Hypothalamic apelin/reactive oxygen species signaling controls hepatic glucose metabolism in the onset of diabetes.下丘脑阿片肽/活性氧信号通路在糖尿病发病过程中控制肝脏葡萄糖代谢。
Antioxid Redox Signal. 2014 Feb 1;20(4):557-73. doi: 10.1089/ars.2013.5182. Epub 2013 Sep 18.
4
Glycogen synthesis in amphibian oocytes: evidence for an indirect pathway.两栖类卵母细胞中的糖原合成：间接途径的证据。
Biochem J. 1996 Apr 15;315 ( Pt 2)(Pt 2):455-60. doi: 10.1042/bj3150455.
5
Characterization of neutral endopeptidase 24.11 in dog glomeruli.犬肾小球中中性内肽酶24.11的特性研究
Biochem J. 1993 May 1;291 ( Pt 3)(Pt 3):773-9. doi: 10.1042/bj2910773.
6
Activation and inactivation of phosphoenolpyruvate carboxykinase by ferrous ions.亚铁离子对磷酸烯醇式丙酮酸羧激酶的激活与失活作用
Biochem J. 1980 Feb 1;185(2):451-4. doi: 10.1042/bj1850451.
7
Indoleamine 2,3-dioxygenase. A new, rapid, sensitive radiometric assay and its application to the study of the enzyme in rat tissues.吲哚胺2,3-双加氧酶。一种新的、快速、灵敏的放射性测定法及其在大鼠组织中该酶研究中的应用。
Biochem J. 1980 Sep 1;189(3):461-6. doi: 10.1042/bj1890461.
8
[14C]bicarbonate fixation into glucose and other metabolites in the liver of the starved rat under halothane anaesthesia. Metabolic channelling of mitochondrial oxaloacetate.在氟烷麻醉下饥饿大鼠肝脏中[14C]碳酸氢盐向葡萄糖及其他代谢产物的固定。线粒体草酰乙酸的代谢通道。
Biochem J. 1985 May 1;227(3):851-65. doi: 10.1042/bj2270851.
9
The effects of stress and injury on the activity of phosphoenolpyruvate carboxykinase in the liver of the rat.应激和损伤对大鼠肝脏中磷酸烯醇丙酮酸羧激酶活性的影响。
Biochem J. 1986 Jan 1;233(1):239-44. doi: 10.1042/bj2330239.
10
The characteristics and site of inhibition of gluconeogenesis in rat liver cells by bacterial endotoxin. Stimulation of phosphofructokinase-1.细菌内毒素对大鼠肝细胞糖异生作用的抑制特性及部位。磷酸果糖激酶-1的激活。
Biochem J. 1987 Mar 15;242(3):721-8. doi: 10.1042/bj2420721.
本文引用的文献
1
COMPARATIVE STUDIES OF CATALYTIC PROPERTIES OF GUINEA PIG LIVER INTRA- AND EXTRAMITOCHONDRIAL PHOSPHOENOLPYRUVATE CARBOXYKINASES.豚鼠肝脏线粒体内外磷酸烯醇式丙酮酸羧激酶催化特性的比较研究。
Biochemistry. 1965 Apr;4:723-31. doi: 10.1021/bi00880a018.
2
Phosphoenolpyruvate carboxykinase and pyruvate carboxylase in developing rat liver.发育中大鼠肝脏中的磷酸烯醇式丙酮酸羧激酶和丙酮酸羧化酶
Biochem J. 1967 Sep;104(3):866-71. doi: 10.1042/bj1040866.
3
Alteration of rat liver phosphoenolpyruvate carboxykinase activity by L-tryptophan in vivo and metals in vitro.L-色氨酸在体内及金属离子在体外对大鼠肝脏磷酸烯醇式丙酮酸羧激酶活性的影响。
Biochemistry. 1967 Jul;6(7):2120-8. doi: 10.1021/bi00859a033.
4
A paradoxical in vivo effect of L-tryptophan on the phosphoenolpyruvate carboxykinase of rat liver.L-色氨酸对大鼠肝脏磷酸烯醇式丙酮酸羧激酶的体内反常效应。
Biochemistry. 1966 Feb;5(2):563-9. doi: 10.1021/bi00866a023.
5
Studies on the mechanisms underlying adaptive changes in rat liver phosphoenolpyruvate carboxykinase.大鼠肝脏磷酸烯醇式丙酮酸羧激酶适应性变化的潜在机制研究。
Biochemistry. 1966 Feb;5(2):555-62. doi: 10.1021/bi00866a022.
6
Paths of carbon in gluconeogenesis and lipogenesis. IV. Inhibition by L-tryptophan of hepatic gluconeogenesis at the level of phosphoenolpyruvate formation.
J Biol Chem. 1966 Sep 10;241(17):3904-8.
7
8
9
10
Effect of acid-base status on renal and hepatic gluconeogenesis in diabetes and fasting.
Am J Physiol. 1969 May;216(5):1207-12. doi: 10.1152/ajplegacy.1969.216.5.1207.
Zotero 插件