用粘性放线菌的可溶性制剂对人外周血淋巴细胞进行体外免疫球蛋白产生刺激。
In vitro stimulation of immunoglobulin production from human peripheral blood lymphocytes by a soluble preparation of Actinomyces viscosus.
作者信息
Mangan D F, Lopatin D E
出版信息
Infect Immun. 1981 Jan;31(1):236-44. doi: 10.1128/iai.31.1.236-244.1981.
Abstract
In this paper, we report the ability of a soluble, ultrasonicate preparation of Actinomyces viscosus to stimulate in vitro immunoglobulin production by human peripheral blood lymphocytes. Immunoglobulin A (IgA), IgG, and IgM concentrations were determined with a highly sensitive and specific micro-enzyme-linked immunosorbent assay (micro-ELISA). A viscosus-induced immunoglobulin production was T lymphocyte dependent and was inhibited by monocytes. The immunoglobulin response was optimal when A. viscosus (1,000 microgram/ml) was removed from the cultures on day 2 of incubation. Antibodies specific for A. viscosus were not detected, indicating that A. viscosus was activating a polycolonal B-lymphocyte response. Since periodontal lesions in humans are characterized by the accumulation of a large number of B lymphocytes, the polyclonal activation of these cells by A. viscosus may affect the severity and resolution of the disease.
摘要
在本文中，我们报告了粘性放线菌的一种可溶性超声裂解制剂刺激人外周血淋巴细胞体外产生免疫球蛋白的能力。采用高度灵敏且特异的微量酶联免疫吸附测定法（微量ELISA）测定免疫球蛋白A（IgA）、IgG和IgM的浓度。粘性放线菌诱导的免疫球蛋白产生依赖于T淋巴细胞，并受到单核细胞的抑制。当在培养的第2天从培养物中去除粘性放线菌（1000微克/毫升）时，免疫球蛋白反应最为理想。未检测到针对粘性放线菌的特异性抗体，这表明粘性放线菌正在激活多克隆B淋巴细胞反应。由于人类牙周病变的特征是大量B淋巴细胞的积聚，粘性放线菌对这些细胞的多克隆激活可能会影响疾病的严重程度和转归。
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In vitro stimulation of immunoglobulin production from human peripheral blood lymphocytes by a soluble preparation of Actinomyces viscosus.用粘性放线菌的可溶性制剂对人外周血淋巴细胞进行体外免疫球蛋白产生刺激。
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