Okada H, Kida T, Yamagami H
Infect Immun. 1983 Jul;41(1):365-74. doi: 10.1128/iai.41.1.365-374.1983.
Advanced human periodontitis is considered to be a B-cell lesion, but the cellular infiltrate contains several cell types, the distribution of which has not been determined. This experiment was designed to characterize and identify the immunocompetent cells on histological sections and in eluates from diseased human gingiva. Immunoglobulin-bearing cells were detected on histological sections by direct immunofluorescence with F(ab')2 antisera monospecific for human immunoglobulin G (IgG), IgA, or IgM. Plasma cells predominated in the central portion of the lamina propria, with the proportions positive for IgG, IgA, and IgM accounting for 65.2 +/- 9.5, 11.2 +/- 1.1, and 1.3 +/- 1.1% of the total infiltrating cells, respectively. T lymphocytes, identified by indirect immunofluorescence with monoclonal antibody (Leu-1) against human T cells, accounted for 29.3 +/- 10.0% of the total infiltrated cells. Most of the T cells were located subjacent to the pocket epithelium, but there were a few in the central lamina propria. Similarly, Fc receptor-bearing cells detected by EA rosetting and macrophages and monocytes detected by nonspecific esterase staining with alpha-naphthylbutyrate esterase were also localized to the region immediately subjacent to the pocket epithelium. Infiltrated cells were harvested from minced gingival tissue after digestion with collagenase, hyaluronidase, and DNase. The eluates contained 35.3 +/- 6.0% T lymphocytes, 30.0 +/- 14.9% Fc receptor-bearing cells, and 12.9 +/- 4.4% monocytes and macrophages. Whereas T gamma cells comprised 13.3 +/- 1.4% of peripheral blood T cells, they accounted for only 6.0 +/- 2.0% of the eluate T cells. In contrast, T mu cells accounted for 44.7 +/- 4.9% of the T cells in the eluates and 51.6 +/- 4.4% in the peripheral blood. The decreased proportion of T gamma cells in the gingiva may indicate a form of abnormal immune regulation concerned with T suppression of B-cell proliferation.
重度人类牙周炎被认为是一种B细胞病变，但细胞浸润包含多种细胞类型，其分布尚未确定。本实验旨在对患病人类牙龈组织切片及洗脱液中的免疫活性细胞进行表征和鉴定。通过用对人免疫球蛋白G（IgG）、IgA或IgM具有单特异性的F(ab')2抗血清进行直接免疫荧光检测，在组织切片上检测到了携带免疫球蛋白的细胞。浆细胞在固有层中部占主导地位，IgG、IgA和IgM阳性的比例分别占总浸润细胞的65.2±9.5%、11.2±1.1%和1.3±1.1%。用抗人T细胞单克隆抗体（Leu-1）通过间接免疫荧光鉴定的T淋巴细胞占总浸润细胞的29.3±10.0%。大多数T细胞位于袋上皮下方，但固有层中部也有少数。同样，通过EA玫瑰花结检测到的携带Fc受体的细胞以及用α-萘丁酸酯酶进行非特异性酯酶染色检测到的巨噬细胞和单核细胞也定位于紧邻袋上皮的区域。用胶原酶、透明质酸酶和DNA酶消化后，从切碎的牙龈组织中收获浸润细胞。洗脱液中含有35.3±6.0%的T淋巴细胞、30.0±14.9%的携带Fc受体的细胞以及12.9±4.4%的单核细胞和巨噬细胞。虽然Tγ细胞在外周血T细胞中占13.3±1.4%，但它们在洗脱液T细胞中仅占6.0±2.0%。相反，Tμ细胞在洗脱液中的T细胞中占44.7±4.9%，在外周血中占51.6±4.4%。牙龈中Tγ细胞比例的降低可能表明存在一种与T细胞抑制B细胞增殖有关的异常免疫调节形式。
