培养的人外周血淋巴细胞产生免疫球蛋白的动力学
Kinetics of immunoglobulin production by cultured human peripheral blood lymphocytes.
作者信息
McLachlan S M, Smith B R, Hall R
出版信息
J Immunol Methods. 1978;21(3-4):211-6. doi: 10.1016/0022-1759(78)90147-3.
A study of immunoglobulin synthesis by human peripheral blood lymphocytes cultured in Marbrook flasks is described. Maximal amounts of immunoglobulin were produced by 3 weeks and most of this was synthesised between 6 and 21 days. In the region of 90% of the immunoglobulin was secreted into the medium. Pokeweed mitogen (PWM) stimulated cultures produced about 10 times as much immunoglobulin as cells cultured in medium only. After 21 days stimulated cultures produced about 150 microgram of IgG, 125 microgram of IgM and 50 microgram of IgA per 10(7) cells.
本文描述了一项关于在Marbrook培养瓶中培养的人外周血淋巴细胞免疫球蛋白合成的研究。3周时产生的免疫球蛋白量达到最大，其中大部分是在6至21天之间合成的。约90%的免疫球蛋白分泌到培养基中。美洲商陆丝裂原（PWM）刺激的培养物产生的免疫球蛋白量约为仅在培养基中培养的细胞的10倍。21天后，刺激的培养物每10⁷个细胞产生约150微克的IgG、125微克的IgM和50微克的IgA。
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