Maquart F X, Vieillard A, Randoux A, Borel J P
Ann Biol Clin (Paris). 1978;36(2):75-7.
A microtechnique of evaluation for the haemoglobins A2 and F is presented, in which the final measurement is made more sensitive by evaluation of peroxidatic activity. Haemoglobin A2 is previously separated by chromatography on a very small column of DEAE cellulose and haemoglobin F is obtained through a microtechnique of alkaline precipitation. The sensitivity of this evaluation is increased by a factor of 20.25 microliter of hemolyzate are enough for a determination. Results obtained are in accordance with those given by the classical methods.
本文介绍了一种评估血红蛋白A2和F的微技术，其中通过评估过氧化物酶活性使最终测量更灵敏。血红蛋白A2事先在一小柱DEAE纤维素上通过色谱法分离，血红蛋白F通过碱性沉淀微技术获得。这种评估的灵敏度提高了20倍。25微升的溶血产物就足以进行一次测定。所得结果与经典方法给出的结果一致。
