Phillips R A
J Supramol Struct. 1980;14(1):77-83. doi: 10.1002/jss.400140108.
Mature, functional lymphocytes rapidly disappear from long-term cultures of mouse bone marrow cells and never reappear. One reason for the loss of B lymphocytes is that the optimal culture conditions for maintenance of myeloid stem cells are suboptimal for lymphocyte survival. However, despite the absence of functional lymphocytes, stem cells from such cultures retain the ability to reconstitute irradiated mice with mitogen-responsive B and T lymphocytes. In fact, in vitro grown stem cells repopulate the lymphoid system better than the myeloid system; the defective myeloid potential does not result from the absence in the cultures of Thy--1 bearing regulatory cells (TSRC). Although the cultures lack mature lymphocytes, they contain putative T cell precursors detectable with an in vitro colony-forming assay (CFU-T). In vitro maintenance of CFU-T requires an appropriate adherent monolayer. Monolyaters from congenitally anemic mice of genotype Sl/Sld fail to support either myeloid precursors or CFU-T.
成熟的功能性淋巴细胞会迅速从小鼠骨髓细胞的长期培养物中消失，且不会再次出现。B淋巴细胞丢失的一个原因是，维持髓系干细胞的最佳培养条件对淋巴细胞存活而言并非最适宜。然而，尽管缺乏功能性淋巴细胞，但此类培养物中的干细胞仍保留了用有丝分裂原反应性B和T淋巴细胞重建受辐照小鼠的能力。事实上，体外培养的干细胞在重建淋巴系统方面比髓系系统表现更好；髓系潜能缺陷并非源于培养物中缺乏表达Thy-1的调节细胞（TSRC）。虽然培养物中缺乏成熟淋巴细胞，但含有可通过体外集落形成试验（CFU-T）检测到的假定T细胞前体。CFU-T的体外维持需要合适的贴壁单层细胞。基因型为Sl/Sld的先天性贫血小鼠的单层细胞无法支持髓系前体或CFU-T。
