Bell-Sakyi Lesley, Růzek Daniel, Gould Ernest A
The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK.
Exp Appl Acarol. 2009 Nov;49(3):209-19. doi: 10.1007/s10493-009-9258-y. Epub 2009 Mar 1.
Primary cell cultures (n = 16) were initiated from tissues of embryonic and neonatal larval Ornithodoros moubata following methods developed for hard ticks. After maintenance for 20-25 months in vitro, cell multiplication commenced in surviving cultures, leading to the establishment of six cell lines designated OME/CTVM21, 22, 24, 25, 26 and 27. All lines are maintained at 28 degrees C, with subculture at 2-8 week intervals. The cultures comprise heterogeneous populations of large cells of 15-100 microm in diameter, often with finger-like protrusions and/or intracellular crystals, rarely attached, predominantly floating and forming clumps or hollow multicellular vesicles up to 1 mm in diameter. Attempts to cryopreserve the cells are described. Tick-borne encephalitis virus has been serially passaged ten times in OME/CTVM21 cells without significant decrease in virus production and with no change in its biological properties as shown by the size and morphology of plaques produced in porcine kidney cells.
原代细胞培养（n = 16）按照为硬蜱开发的方法，从胚胎期和新生幼虫期的莫氏钝缘蜱组织中开始建立。在体外培养20 - 25个月后，存活培养物中开始出现细胞增殖，从而建立了六个细胞系，分别命名为OME/CTVM21、22、24、25、26和27。所有细胞系均在28℃下保存，每隔2 - 8周传代一次。培养物由直径为15 - 100微米的大细胞异质群体组成，这些细胞通常有指状突起和/或细胞内晶体，很少附着，主要呈漂浮状态，并形成直径达1毫米的团块或中空多细胞囊泡。文中描述了对细胞进行冷冻保存的尝试。蜱传脑炎病毒已在OME/CTVM21细胞中连续传代十次，病毒产量没有显著下降，并且其生物学特性没有改变，这通过在猪肾细胞中产生的噬斑大小和形态得以证明。
