Boguta G, Dancewicz A M
Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Feb;39(2):163-74. doi: 10.1080/09553008114550181.
Products of radiolysis of tyrosine and glycyl-L-tyrosine in oxygen-free nitrogen, N2O or air saturated water solution, pH 4.0 or 8.6 were analysed in an amino acid analyser and upon separation on DEAE cellulose or BioGel P-2 column with spectrofluorimetry. Apart from dihydroxyphenylalanine tyrosine solution irradiated in nitrogen or N2O contained dityrosine, while that of irradiated glycyl-L-tyrosine contained glycyl-dityrosine-glycine. Both dimeric products were formed with radiation yields, of about 0.15 at low pH value. In addition an unknown, nonfluorescent but ninhydrin positive product was found in irradiated tyrosine solution. Another, unidentified product of radiolysis was detected in N2O or air saturated solutions of tyrosine. The product had a blue-green fluorescence and a molecular weight of about 500.
在无氧氮气、一氧化二氮或空气饱和水溶液（pH 4.0或8.6）中，酪氨酸和甘氨酰-L-酪氨酸的辐射分解产物在氨基酸分析仪中进行分析，并在DEAE纤维素或BioGel P-2柱上分离后用荧光光谱法进行检测。除了二羟基苯丙氨酸外，在氮气或一氧化二氮中辐照的酪氨酸溶液含有二酪氨酸，而辐照的甘氨酰-L-酪氨酸溶液含有甘氨酰-二酪氨酸-甘氨酸。在低pH值下，这两种二聚体产物的辐射产率均约为0.15。此外，在辐照的酪氨酸溶液中发现了一种未知的、无荧光但茚三酮呈阳性的产物。在一氧化二氮或空气饱和的酪氨酸溶液中检测到另一种未鉴定的辐射分解产物。该产物具有蓝绿色荧光，分子量约为500。
