Szczylik C, Wiktor-Jedrzejczak W
Int J Radiat Biol Relat Stud Phys Chem Med. 1981 Mar;39(3):253-63. doi: 10.1080/09553008114550331.
Human peripheral blood lymphocytes isolated using a Ficoll-Uropolinum gradient were exposed to between 1 and 25 Gy X-rays and were cultured for period of up to 72 hours. Before irradiation and after 2, 24, 48 and 72 hours total numbers of surviving cells per ml of culture were determined as well as the frequencies of cells spontaneously forming rosettes (E) and active rosettes (AE) with sheep red blood cells, cells bearing receptor for activated complement (EAC) and cells spontaneously forming rosettes with mouse red blood cells (ME). Irrespective of the duration of in vitro culture, the radiosensitivity of the subpopulations of human lymphocytes increased in the order E less than EAC = ME less than AE rosette forming cells. Calculation of the frequencies of surviving cells, null with respect to the surface markers (E and EAC), showed that disappearence of rosette-forming cells under the influence of radiation was largely due to the shedding of their receptors and not exclusively to cell death. It is suggested that the extreme radiosensitivity of the subpopulation of T lymphocytes capable of AE rosette formation, if confirmed by in vivo studies, may possess diagnostic significance in persons accidentally exposed to radiation.
使用Ficoll-尿嘧啶梯度分离的人外周血淋巴细胞接受1至25 Gy的X射线照射，并培养长达72小时。在照射前以及照射后2、24、48和72小时，测定每毫升培养物中存活细胞的总数，以及与绵羊红细胞自发形成玫瑰花结（E）和活性玫瑰花结（AE）的细胞频率、带有活化补体受体的细胞（EAC）以及与小鼠红细胞自发形成玫瑰花结的细胞（ME）。无论体外培养的持续时间如何，人淋巴细胞亚群的放射敏感性按E＜EAC = ME＜AE玫瑰花结形成细胞的顺序增加。计算相对于表面标志物（E和EAC）为零的存活细胞频率表明，辐射影响下玫瑰花结形成细胞的消失很大程度上是由于其受体的脱落，而不仅仅是细胞死亡。有人提出，如果体内研究证实能够形成AE玫瑰花结的T淋巴细胞亚群具有极高的放射敏感性，那么对于意外受辐射的人员可能具有诊断意义。
