Prosser J S
Int J Radiat Biol Relat Stud Phys Chem Med. 1976 Nov;30(5):459-65. doi: 10.1080/09553007614551271.
The survival of unstimulated human T and B lymphocytes after X-irradiation in vitro was measured by Trypan Blue dye exclusion over a period of four days. B cell numbers were observed to decline rapidly even after relatively low doses, but T cell numbers fell much more slowly. A comparison of the percentage survival 96 hours after irradiation shows that in this system T cells are between approximately 2 and 5 times more resistant than B cells. Data for interphase death after 48 hours are compared with cytogenetic data for interphase loss of PHA-stimulated human lymphocytes and are shown to be in broad agreement at radiation doses below 400 rad. It is suggested that at higher doses mitotic delay may be increasingly important leading to selection of non-irradiated cells at 48 hours.
通过台盼蓝拒染法在四天时间内测定了体外X射线照射后未受刺激的人T淋巴细胞和B淋巴细胞的存活率。即使在相对低剂量照射后，也观察到B细胞数量迅速下降，但T细胞数量下降得要慢得多。对照射后96小时存活百分比的比较表明，在该系统中T细胞的抗性比B细胞大约高2至5倍。将48小时后间期死亡的数据与PHA刺激的人淋巴细胞间期丢失的细胞遗传学数据进行比较，发现在辐射剂量低于400拉德时两者大致相符。有人提出，在较高剂量下，有丝分裂延迟可能变得越来越重要，导致在48小时时选择未受照射的细胞。
