Abd-el-Fattah M, Scherer R, Fouad F M, Ruhenstroth-Bauer G
Cancer Res. 1981 Jun;41(6):2548-55.
Transplantation of Yoshida sarcoma (solid type) and Zajdela ascites hepatoma tumors in rats induces a biphasic change in the concentration of the following five acute-phase proteins: alpha-1-acid glycoprotein; alpha-1-antitrypsin; haptoglobin; hemopexin; and ceruloplasmin. These proteins and other plasma proteins were quantitated by two-dimensional immunoelectrophoresis relative to normal serum concentrations. The elevation of most of these acute-phase proteins was greater in the second phase, during which serum levels increased continuously as the tumor burden increased until the animals died. The increase in haptoglobin concentration during the second phase was much higher in rats bearing Yoshida sarcoma than in rats bearing Zajdela tumors. Rats receiving irradiated tumor cells showed neither tumor growth nor second-phase protein changes. Significant increases in uptake of 3H-amino acids by isolated perfused livers of tumor-bearing rats provided evidence for an increase in the hepatic synthesis rates of the acute-phase proteins. Removal of the solid tumor resulted in a gradual decrease of acute-phase protein concentrations with concomitant increase in serum albumin concentration. These alterations in serum acute-phase proteins during tumor growth and after removal of the tumor may make their use attractive as biological markers of the response of the tumor-bearing animal to its tumor.
在大鼠中移植吉田肉瘤（实体型）和扎伊德拉腹水肝癌会导致以下五种急性期蛋白浓度出现双相变化：α-1-酸性糖蛋白、α-1-抗胰蛋白酶、触珠蛋白、血红素结合蛋白和铜蓝蛋白。相对于正常血清浓度，通过二维免疫电泳对这些蛋白质和其他血浆蛋白进行定量。这些急性期蛋白中的大多数在第二阶段升高幅度更大，在此期间，随着肿瘤负荷增加，血清水平持续升高，直至动物死亡。在第二阶段，携带吉田肉瘤的大鼠中触珠蛋白浓度的增加比携带扎伊德拉肿瘤的大鼠高得多。接受辐照肿瘤细胞的大鼠既无肿瘤生长，也无第二阶段的蛋白质变化。荷瘤大鼠离体灌注肝脏对³H-氨基酸摄取的显著增加为急性期蛋白肝脏合成率增加提供了证据。切除实体瘤导致急性期蛋白浓度逐渐降低，同时血清白蛋白浓度升高。肿瘤生长期间和切除肿瘤后血清急性期蛋白的这些变化可能使其作为荷瘤动物对肿瘤反应的生物学标志物具有吸引力。
